AI Article Synopsis
- Developed and validated direct and indirect HPLC-UV methods for measuring anthraquinones in dried madder root.
- The direct method uses ethanol-water extraction to identify major glycosides, while the indirect method converts glycosides into aglycones before extraction.
- Indirect extraction is simpler and useful for determining key anthraquinones, such as alizarin and nordamnacanthal, with a proportional relationship to lucidin primeveroside levels.
- Various madder cultivars were analyzed for their anthraquinone content.
Article Abstract
Direct and indirect HPLC-UV methods for the quantitative determination of anthraquinones in dried madder root have been developed, validated and compared. In the direct method, madder root was extracted twice with refluxing ethanol-water. This method allowed the determination of the two major native anthraquinone glycosides lucidin primeveroside and ruberythric acid. In the indirect extraction method, the anthraquinone glycosides were first converted into aglycones by endogenous enzymes and the aglycones were subsequently extracted with tetrahydrofuran-water and then analysed. In this case the anthraquinones alizarin, purpurin and nordamnacanthal may be determined. The content of nordamnacanthal is proportional to the amount of lucidin primeveroside originally present. The indirect extraction method is easier to apply. Different madder cultivars were screened for their anthraquinone content.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/pca.800 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Genome sequence of Fusarium oxysporum strain ByF01, the causal agent of root rot of Knoxia roxburghii in China.
BMC Genom Data
June 2024
Institute of Medicinal Plant Cultivation, School of Chinese Materia Medica, Academy of Southern Medicine, Yunnan University of Chinese Medicine, Kunming, 650500, China.
Objectives: Knoxia roxburghii is a member of the madder (Rubiaceae) family. This plant is cultivated in different areas of China and recognized for its medicinal properties, which leads to its use in traditional Chinese medicine. The incidence of root rot was 10-15%.
View Article and Find Full Text PDFA prenyltransferase participates in the biosynthesis of anthraquinones in Rubia cordifolia.
Plant Physiol
July 2024
State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, P.R. China.
Anthraquinones (AQs) constitute the largest group of natural quinones, which are used as safe natural dyes and have many pharmaceutical applications. In plants, AQs are biosynthesized through two main routes: the polyketide pathway and the shikimate pathway. The latter primarily forms alizarin-type AQs, and the prenylation of 1,4-dihydroxy-2-naphthoic acid (DHNA) is the first pathway-specific step.
View Article and Find Full Text PDFEnvironmental friendly utilization of plant wastes in combination as a source of natural colorants for binary mordanted silk dyeing.
Environ Sci Pollut Res Int
November 2023
Department of Applied Chemistry, Government College University Faisalabad, Faisalabad, 38000, Pakistan.
This study investigates the absorption behavior of natural dyes on silk fabric: madder roots (red dye) and amaltas (brown dye). The dyes were extracted under optimized conditions in an acidic medium. Binary mixtures of these dyes in different proportions were employed to develop various shades.
View Article and Find Full Text PDFSite-specific genotoxicity of rubiadin: localization and histopathological changes in the kidneys of rats.
Arch Toxicol
December 2023
Division of Pathology, National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-Ku, Kawasaki-Shi, Kanagawa, 210-9501, Japan.
Rubiadin (Rub) is a genotoxic component of madder color (MC) that is extracted from the root of Rubia tinctorum L. MC induces renal tumors and preneoplastic lesions that are found in the proximal tubule of the outer stripe of the outer medulla (OSOM), suggesting that the renal carcinogenicity of MC is site specific. To clarify the involvement of Rub in renal carcinogenesis of MC, we examined the distribution of Rub in the kidney of male gpt delta rats that were treated with Rub for 28 days.
View Article and Find Full Text PDFFunctional immune boosters; the herb or its dead microbiome? Antigenic TLR4 agonist MAMPs found in 65 medicinal roots and algae's.
J Funct Foods
August 2023
Florida Agricultural and Mechanical University, College of Pharmacy and Pharmaceutical Sciences, Tallahassee, FL 32307, United States.
Background: Humans have been consuming medicinal plants (as herbs/ spices) to combat illness for centuries while ascribing beneficial effects predominantly to the plant/phytochemical constituents, without recognizing the power of obligatory resident microorganism' communities (MOCs) (live/dead bacteria, fungus, yeast, molds etc.) which remain after industrial microbial reduction methods. Very little is known about the taxonomic identity of residual antigenic microbial associated molecular patterns (MAMPs) debris in our botanical over the counter (OTC) products, which if present would be recognized as foreign (non-self) antigenic matter by host pattern recognition receptors (PRRs) provoking a host immune response; this the basis of vaccine adjuvants.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!