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Electrical asymmetrical flow field-flow fractionation: Fundamentals, evolution, applications, and prospects.
J Chromatogr A
January 2025
Department of Analytical Chemistry and Food Technology, Environmental Sciences Institute (ICAM), University of Castilla-La Mancha, Avda. Carlos III s/n, 45071 Toledo, Spain. Electronic address:
Among the plethora of techniques that conforms the Field-Flow Fractionation (FFF) family, electrical field-flow fractionation (ElFFF) was designed to separate different analytes based on their size and electrophoretic mobility (µ). However, major technical and operational issues made this technique to fall into oblivion. Many of those drawbacks can be circumvented if another field is employed as the main driving force for the elution in the same channel, such as the most successful and useful FFF-related technique, asymmetrical flow field-flow fractionation (AF4).
View Article and Find Full Text PDFMultilevel modeling and control of dynamic systems.
Sci Rep
November 2024
Software Engineering Department, Braude College of Engineering, Karmiel, Israel.
The underlying changes of complex dynamic systems are affected by numerous highly interdependent components with nonlinear interactions and cannot be suitably predicted by merely analyzing their individual parts. Recent technological developments (reducing the size of actuators and sensors, increasing speed and productivity) make it possible to monitor and handle systems on time, even during transient processes. In many practical applications, to effectively study rapidly evolving systems, their structure has to be examined at three levels: Micro-Scale (Short-Term), Meso-Scale (Intermediate-Term), and Macro-Scale (Long-Term).
View Article and Find Full Text PDF[High stability enhanced ultrasonic microfluidic structure with flexible tip coupled bubbles].
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi
October 2024
Key Lab of Microelectronic Devices & Integrated Technology, Institute of Microelectronics, Chinese Academy of Sciences, Beijing 100029, P. R. China.
Ultrasonic microfluidic technology is a technique that couples high-frequency ultrasonic excitation to microfluidic chips. To improve the issues of poor disturbance effects with flexible tip structures and the susceptibility of bubbles to thermal deformation, we propose an enhanced ultrasonic microchannel structure that couples flexible tips with bubbles aiming to improve the disturbance effects and the stability duration. Firstly, we used finite element analysis to simulate the flow field distribution characteristics of the flexible tip, the bubble, and the coupling structure and obtained the steady-state distribution characteristics of the velocity field.
View Article and Find Full Text PDFMicro scale chromatography of human plasma proteins for nano LC-ESI-MS/MS.
Anal Biochem
February 2025
Research Analytical Biochemistry Laboratory, Department of Chemistry and Biology, Toronto Metropolitan University, Canada. Electronic address:
Organic precipitation of proteins with acetonitrile demonstrated complete protein recovery and improved chromatography of human plasma proteins. The separation of 25 μL of human plasma into 22 fractions on a QA SAX resin facilitated more effective protein discovery despite the limited sample size. Micro chromatography of plasma proteins over quaternary amine (QA) strong anion exchange (SAX) resins performed best, followed by diethylaminoethyl (DEAE), heparin (HEP), carboxymethyl cellulose (CMC), and propyl sulfate (PS) resins.
View Article and Find Full Text PDFMicro-Scale Ice Shoveling Effect Induced by Magnetic-Responsive Microfins.
Adv Sci (Weinh)
December 2024
Beijing Institute of Technology, Zhuhai Beijing Institute of Technology (BIT), Zhuhai, 519088, China.
Icing is ubiquitous in nature and engineering applications, and imposes threats to road and air transportations, wind energy infrastructures, etc. However, current active de-icing solutions, especially the most popular one, i.e.
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