[Immunoscreening of newborn larvae cDNA library of Trichinella spiralis].

Objective: To obtain antigenic genes encoding newborn larvae antigens of Trichinella spiralis.

Methods: Newborn larvae (NBL) cDNA library of Trichinella spiralis was screened using the infected swine serum and positive clones were sequenced and analysed.

Results: 158 positive clones were obtained from 4.5 x 10(5) recombinant clones by immunoscreening. The sequence analysis of positive clones showed that there are 36 novel cDNAs, 5 reported cDNAs and 12 cDNAs without the ORF showing high similarity to the mitochondrial DNA of Trichinella spiralis.

Conclusion: Some cDNAs encoding antigenic protein of newborn larvae of Trichinella spiralis were obtained.