Objective: To make high efficiency expression of the SAG2 gene from Toxoplasma gondii RH strain in E. coli and study the antigenicity of the expressed product.
Methods: The SAG2 gene fragment of T. gondii RH strain amplified by PCR method from genome DNA was cloned into the pMD-18T vector and transformed into E. coli DH5alpha. After nucleotide sequencing, the SAG2 gene fragment was subcloned into the expression vector pET23a with correct orientation and transformed into E. coli DH5alpha. The plasmid from the correct clone identified by PCR method and endonuclease digestion was transformed into E. coli BL21 (DE3) and induced for expression. The expressed product was studied by SDS-PAGE and Western blot.
Results: 502 bp gene fragment was amplified by PCR as anticipated. Nucleotide sequencing showed a 100% homology with that of the published sequence in GenBank. The molecular weight of the expressed protein was about Mr 19,000. Western blotting indicated that the antigenicity of the protein was specific.
Conclusion: The plasmid pET23a-SAG2 was constructed and a high efficiency expression of the SAG2 gene from T. gondii RH strain was made. The expressed product shows a specific antigenicity.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
EVALUATION OF GRA6 AS GENETIC MARKER FOR DETERMINING GENOTYPE IN THE CEREBROSPINAL FLUID OF HIV/AIDS PATIENTS WITH TOXOPLASMIC ENCEPHALITIS.
Afr J Infect Dis
October 2024
Department of Parasitology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia.
Background: Toxoplasmic encephalitis is a severe manifestation of infection, with potentially fatal outcomes, particularly among immunocompromised patients. Clinical manifestation of this infection is associated with a specific genotype of , requiring the use of genetic marker for genotype determination.
Aims: This study critically evaluated the application of GRA6 gene as genetic marker for genotyping in cerebrospinal fluid (CSF) samples from HIV/AIDS patients diagnosed with Toxoplasmic encephalitis.
Comparative GRA6 and GRA7 for their Utility as Genetic Markers in the Genotyping of Cerebral Toxoplasmosis in Cerebrospinal Fluid.
Acta Parasitol
September 2024
Department of Parasitology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia.
Introduction: Cerebral toxoplasmosis is a severe symptom of Toxoplasma gondii (T. gondii) infection that often affects individuals with Human Immunodeficiency Virus (HIV) infection and can be fatal. T.
View Article and Find Full Text PDFFirst Sero-Molecular Diagnosis of Toxoplasma gondii and Toxocara spp. Infections in the Police Dogs and Their Trainers in Iran.
Acta Parasitol
September 2024
Institute of Police Equipment and Technologies, Policing Sciences and Social Studies Research Institute, Tehran, Iran.
Article Synopsis
- - The study aimed to evaluate the prevalence of Toxoplasma gondii and Toxocara spp. infections among police dogs and their trainers in Tehran, Iran, since no prior studies had been conducted in this area.
- - Researchers collected 150 samples consisting of blood and fecal samples from police dogs and blood samples from their trainers, using various tests such as the Modified Agglutination Test (MAT) and molecular techniques to detect specific parasites.
- - The findings indicated a prevalence of T. gondii at 4% in dogs and 10% in trainers, and Toxocara spp. was found in 6% of trainer samples; there were no significant associations between infections and the examined variables. *
First identified Toxoplasma gondii Type I in market-sold ducks in Fujian province, China: a significant for public health.
Poult Sci
September 2024
Key Laboratory of Fujian-Taiwan Animal Pathogen Biology, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address:
Article Synopsis
- * Out of 586 ducks tested, 35 were found positive for the T. gondii B1 gene, and one heart sample was identified as Type I (ToxoDB#10) for the first time in ducks in China.
- * The research adds important information about the genetic diversity of T. gondii in free-range ducks, highlighting its relevance to the "One Health" concept connecting human, animal, and environmental health.
Molecular Models of in Humans and Animals.
Turkiye Parazitol Derg
June 2024
Çankırı Karatekin Üniversitesi Sağlık Bilimleri Fakültesi, Çankırı, Türkiye.
() is an obligate intracellular, zoonotic protozoan parasite of interest to physicians and veterinarians with its highly complex structure. It is known to infect about one-third of the world's population. Since it is a zoonotic disease, it is necessary to keep the animal population under control in order to prevent human exposure.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!