Objective: To establish and optimize the two-dimensional electrophoresis (2-DE) of uterine leiomyoma for the proteome analysis.
Methods: Run immobilized pH gradient (IPG)-isoelectric focusing electrophoresis as the first dimension, then vertical SDS-PAGE electrophoresis as the second dimension. A series of important steps,such as sample solubility, volume of loading, electrophoresis parameters and protocol for staining were optimized.
Results: The 2-DE patterns of uterine leiomyoma and myometrium with good quality were obtained.
Conclusion: With optimal condition the two-dimensional electrophoresis of uterine leiomyoma can be obtained.
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| http://dx.doi.org/10.3785/j.issn.1008-9292.2004.06.018 | DOI Listing |
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