Although mitotic and meiotic spindles maintain a steady-state length during metaphase, their antiparallel microtubules slide toward spindle poles at a constant rate. This "poleward flux" of microtubules occurs in many organisms and may provide part of the force for chromosome segregation. We use quantitative image analysis to examine the role of the kinesin Eg5 in poleward flux in metaphase Xenopus laevis egg extract spindles. Pharmacological inhibition of Eg5 results in a dose-responsive slowing of flux, and biochemical depletion of Eg5 significantly decreases the flux rate. Our results suggest that ensembles of nonprocessive Eg5 motors drive flux in metaphase Xenopus extract spindles.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172449 | PMC |
| http://dx.doi.org/10.1083/jcb.200407126 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
HistoNeXt: dual-mechanism feature pyramid network for cell nuclear segmentation and classification.
BMC Med Imaging
January 2025
Department of Information, Third Affiliated Hospital of Naval Medical University, No. 225 Changhai Road, Yangpu District, 200438, Shanghai, China.
Purpose: To develop an end-to-end convolutional neural network model for analyzing hematoxylin and eosin(H&E)-stained histological images, enhancing the performance and efficiency of nuclear segmentation and classification within the digital pathology workflow.
Methods: We propose a dual-mechanism feature pyramid fusion technique that integrates nuclear segmentation and classification tasks to construct the HistoNeXt network model. HistoNeXt utilizes an encoder-decoder architecture, where the encoder, based on the advanced ConvNeXt convolutional framework, efficiently and accurately extracts multi-level abstract features from tissue images.
Research progress of paclitaxel nanodrug delivery system in the treatment of triple-negative breast cancer.
Mater Today Bio
December 2024
State Key Laboratory of Research & Development of Characteristic Qin Medicine Resources (Cultivation), and Shaanxi Province Key Laboratory of New Drugs and Chinese Medicine Foundation Research, Shaanxi University of Chinese Medicine, Xi'an, 712046, China.
Triple-negative breast cancer (TNBC) is the most aggressive subtype of breast cancer, characterized by the loss or low expression of estrogen receptor (ER), human epidermal growth factor receptor 2 (HER2) and progesterone receptor (PR). Due to the lack of clear therapeutic targets, paclitaxel (PTX) is often used as a first-line standard chemotherapy drug for the treatment of high-risk and locally advanced TNBC. PTX is a diterpenoid alkaloid extracted and purified from Taxus plants, functioning as an anticancer agent by inducing and promoting tubulin polymerization, inhibiting spindle formation in cancer cells, and preventing mitosis.
View Article and Find Full Text PDFCross-speed spindle motor bearings fault diagnosis combined with multi-space variable scale adaptive filter and feedforward hybrid strategy.
ISA Trans
November 2024
National Numerical Control System Engineering Research Center, Huazhong University of Science and Technology, Mechanical Building, 1037 Luoyu Road, Wuhan 430074, China. Electronic address:
The vibration signal of the spindle motor contains complicated mixed modulation harmonics and background noise when computer numerical control (CNC) machine tools perform machining tasks. Additionally, frequent changes in the running speed of the spindle motor cause significant variations in the signal feature distribution, making fault diagnosis challenging. The adaptive sinusoidal fusion convolutional neural networks (ASFCNN) is proposed to achieve cross-speed spindle motor bearings fault diagnosis.
View Article and Find Full Text PDFDifferences in brain spindle density during sleep between patients with and without type 2 diabetes.
Comput Biol Med
January 2025
Biostatistics and Computational Biology Branch, National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA. Electronic address:
Article Synopsis
- Sleep spindles, which may play a role in glucose regulation, were significantly lower in patients with type 2 diabetes mellitus (T2DM) compared to healthy individuals.
- A study of 952 T2DM patients and 952 matched controls revealed that T2DM patients had about half the spindle density during sleep, and this trend was confirmed in additional data from the Sleep Heart Health Study.
- The reduction in spindle density in T2DM patients was associated with disease severity and glycemic control, suggesting a link between sleep disturbances and diabetes management.
Quantitatively Analyzing the Morphological Growth Dynamics of Bipolar and Non-bipolar Spindles Using Xenopus Egg Extract.
Methods Mol Biol
December 2024
Department of Chromosome Science, National Institute of Genetics, Shizuoka, Japan.
The bipolar shape of the microtubule-based spindle is a pivotal morphological phenotype for accurate chromosome segregation during cell division. However, existing descriptions of spindle morphogenesis remain largely qualitative. Here, we introduce a method that provides a quantitative description of the morphological growth dynamics of spindles using Xenopus egg cytoplasmic extract and a computational image analysis pipeline.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!