1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) was tested for interference with the Lowry method of protein determination. The EDC interference was nearly additive at both 660 and 750 nm. Blue-colored complex developed in the case of EDC and showed maximum absorbance at 750 nm, similar to the bovine serum albumin (BSA) estimation. In time response analysis, blue-colored complex was developed after 30 min of incubation in both cases (EDC and BSA); therefore, it followed the same kinetic pattern of color development as that of BSA. Interference is believed to be caused by the reduction of the folin-ciocalteu reagent by EDC, resulting in increased blue-colored complex and apparently causing increased protein content of the sample.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ab.2004.09.038 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Wide-range tunable circularly polarized luminescence in triphenylamine supramolecular polymers via charge-transfer complexation.
Nat Commun
October 2024
Department of Vascular Surgery, The First Affiliated Hospital of University of Science and Technology of China (USTC), and Key Laboratory of Precision and Intelligent Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, Anhui, PR China.
Article Synopsis
- Researchers developed a method to create circularly polarized luminescence materials with adjustable colors by using charge-transfer complexation in chiral supramolecular polymers.
- By varying the strength of charge transfer between specific molecules, they achieved a wide range of luminescent colors from blue to red, depending on the polymer structure.
- The team also found that mixing different luminescent materials in micelles can produce white-light circularly polarized luminescence, enhancing the potential applications in 3D displays and photonics.
Bimetallic porous Mn/Co oxide nanosheets with efficient oxidase-mimicking activity for the sensitive colorimetric determination of resveratrol in different matrices.
Anal Chim Acta
August 2024
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt. Electronic address:
Background: Resveratrol, a natural polyphenol compound used as an ingredient in dietary supplements, and pharmaceuticals, has gained significant attention due to its potential health benefits. However, the accurate and sensitive determination of resveratrol in complex matrices remains a challenge. In this study, we propose the utilization of bimetallic porous Mn/Co oxide nanosheets (MnCoO-NSs) as catalysts for the colorimetric determination of resveratrol.
View Article and Find Full Text PDFRegulatory gene network for coffee-like color morph of TYRP1 mutant of oujiang color common carp.
BMC Genomics
July 2024
Key Laboratory of Freshwater Aquatic Genetic Resources Certificated By the Ministry of Agriculture and Rural Affairs, Shanghai Engineering Research Center of Aquaculture, National Demonstration Centre for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China.
Background: Neither a TYRP1-mediated highly conserved genetic network underlying skin color towards optimum defense nor the pathological tendency of its mutation is well understood. The Oujiang Color Common Carp (Cyprinus carpio var. color) as a model organism, offering valuable insights into genetics, coloration, aquaculture practices, and environmental health.
View Article and Find Full Text PDFMetal ion-complexed DNA probe coupled with CRISPR/Cas12a amplification and AuNPs for sensitive colorimetric assay of metallothionein in fish.
Spectrochim Acta A Mol Biomol Spectrosc
November 2024
School of Chemistry and Chemical Engineering, Chongqing University of Technology, Chongqing 400054, PR China.
The accurate and sensitive detection of metallothionein (MT) is of great significance in the fields of biomedical, toxicological and environmental sciences. In this work, based on the high affinity interaction between MT and the heavy metal ions of Hg and the significant signal amplification capability of Cas12a/crRNA enzyme as well, we report a simple and highly sensitive method for visual detection of MT, a biomarker in fish for heavy metal ion-induced water bio-pollution. The target MT molecules bind Hg in the Hg- complexed hairpin DNA probes to unfold the hairpin structure into ssDNAs, which hybridize with the partial dsDNA duplexes via strand displacement to yield specific sequence-containing dsDNAs.
View Article and Find Full Text PDFA highly effective peroxidase-mimic nanozyme of S, N-carbon dot-decorated cerium organic framework-based colorimetric detection of Hg ion and thiophanate methyl.
Anal Methods
June 2024
Department of Chemistry, SRM Institute of Science and Technology, Kattankulathur, 603 203, Tamil Nadu, India.
In this study, we proposed a colorimetric probe as S, N-carbon dot-decorated Ce-MOF (S, N-CD@Ce-MOF) for the dual detection of mercury and thiophanate methyl (TM), which are simultaneously present pollutants in the environment and foodstuffs. These pollutants cause serious threats to human health, such as carcinogenicity and neurovirulence. Herein, we synthesized S, N-CD@Ce-MOF using the hydrothermal method and applied it to a "turn-off-on" probe to detect mercury and TM using the colorimetric method in water and food samples.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!