Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC535390 | PMC |
| http://dx.doi.org/10.1073/pnas.0407898101 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Praesto Jetted A50 HipH, a mild pH elution Protein A resin, exhibits improved aggregate separation capability and protein elution from it shows unique response to mobile phase additive sodium chloride.
Protein Expr Purif
January 2025
Downstream Process Development (DSPD), WuXi Biologics, 31 Yiwei Road, Waigaoqiao Free Trade Zone, Shanghai 200131, China. Electronic address:
Protein A affinity chromatography has been widely used for product capture in monoclonal antibody (mAb), bispecific antibody (bsAb) and Fc-fusion protein purification. However, the low pH (i.e.
View Article and Find Full Text PDFRoles of Mature Domain Targeting Signals (MTSs) for Protein Translocation and Secretion in .
Int J Mol Sci
December 2024
Institute of Food Technology, Department of Food Science and Technology, BOKU University, 1190 Vienna, Austria.
is a potential bacterial cell factory to develop delivery systems for vaccines and therapeutic proteins. Much progress has been made in applications using engineered against, e.g.
View Article and Find Full Text PDFDesign of a yeast SUMO tag to eliminate internal translation initiation.
Protein Sci
January 2025
Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio, USA.
After overexpression in a suitable host, recombinant protein purification often relies on affinity (e.g., poly-histidine) and solubility-enhancing (e.
View Article and Find Full Text PDFThe Cwr1 protein kinase localizes to the plasma membrane and mediates resistance to cell wall stress in .
mSphere
December 2024
Department of Microbiology and Immunology, Stony Brook University, Stony Brook, New York, USA.
Unlabelled: The plasma membrane is critical for the virulence of the human fungal pathogen . In addition to functioning as a protective barrier, the plasma membrane plays dynamic roles in a wide range of functions needed for virulence including nutrient uptake, cell wall synthesis, morphogenesis, resistance to stress, and invasive hyphal growth. Screening a collection of mutants identified an understudied gene that is important for invasive hyphal growth, which we have termed (Cell Wall Regulatory kinase).
View Article and Find Full Text PDFRational design of uncleaved prefusion-closed trimer vaccines for human respiratory syncytial virus and metapneumovirus.
Nat Commun
November 2024
Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA, 92037, USA.
Respiratory syncytial virus (RSV) and human metapneumovirus (hMPV) cause human respiratory diseases and are major targets for vaccine development. In this study, we design uncleaved prefusion-closed (UFC) trimers for the fusion protein (F) of both viruses by examining mutations critical to F metastability. For RSV, we assess four previous prefusion F designs, including the first and second generations of DS-Cav1, SC-TM, and 847A.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!