AI Article Synopsis
- This report introduces a new method using p-iodophenol-enhanced luminol chemiluminescence to measure peroxidase activity in certain types of acute myeloblastic leukemia (AML).
- The procedure involved thawing frozen blast cells from 29 patients and applying an optimized protocol for the tests.
- The results indicated that all AML-M7 and AML-MI cases emitted light significantly above a set threshold, suggesting the technique effectively distinguishes these AML types from acute lymphoblastic leukemia (ALL), while it was also successful in identifying most AML-M0 cases.
Article Abstract
Introduction: In this report, we propose the application of the p-iodophenol-enhanced luminol chemiluminescent technique to the determination of peroxidase (myeloperoxidase and/or platelet peroxidase) activity in blasts of minimally differentiated acute myeloblastic leukemia (AML-M0) and acute megakaryoblastic leukemia (AML-M7).
Methods: The frozen blast cells from 29 patients were thawed and submitted to the optimized protocol.
Results: All cases of AML-M7 and AML-M I exhibited integrated light emission greater than 73(10(2) mV x s), which was the arbitrary cutoff point set for the discrimination between AML and acute lymphoblastic leukemia (ALL) (mean + 3 x s.d. of ALL samples, n = 10). In addition, five out of seven cases of AML-MO showed results above the cutoff point.
Conclusion: This highly sensitive enhanced chemiluminescent technique may be applied to discriminate between ALL and AML-M7 or AML-MI cases, and most AML-M0 cases. It is very simple, cheap and easy to perform compared to other procedures used to measure MPO activity in AML-leukemias including AML-M7 and AML-M0.
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| http://dx.doi.org/10.1038/sj.thj.6200562 | DOI Listing |
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