Aim: To explore whether the differential effects of Zn2+ on ryanodine binding to the sarcoplasmic reticulum (SR) of skeletal and cardiac muscles resulted from different permeability of the SR to Zn2+.
Methods: [3H]ryanodine binding assays were performed to examine the effect of Zn2+ on ryanodine binding to the SR in the presence of pyrithione sodium (PyNa), a specific Zn2+ ionophore.
Results: As a control, PyNa up to 50 micromol/L did not induce any effect on ryanodine binding to the SR of cardiac muscle. But PyNa 1-100 micromol/L increased ryanodine binding in skeletal muscle with maximum binding (222.2+/-20.9 % of the control) and inhibited ryanodine binding to 50 % of the control at about 500 micromol/L. In the presence of PyNa 10 and 50 micromol/L the dose-dependence of the effect of Zn2+ in cardiac muscle was still monophasic and not changed by PyNa, while the biphasic effect of Zn2+ in skeletal muscle became monophasic.
Conclusion: Different permeability of the SR to Zn2+ may account for the differential effects of Zn2+ on ryanodine binding in skeletal and cardiac muscles. PyNa is not a strictly specific Zn2+ ionophore.
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