[Biological effect of serum of kidney-tonifying traditional Chinese drug-fed rats on cultured osteoblasts].

Di Yi Jun Yi Da Xue Xue Bao

Department of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.

Published: November 2004

AI Article Synopsis

  • The study aimed to explore how the serum from rats fed with kidney-tonifying traditional Chinese medicine affects cultured osteoblasts.
  • The researchers treated osteoblasts with serum from rats given different doses of the drugs and observed improvements in cell growth, differentiation, and mineralization.
  • Results showed that the serum significantly boosted cell proliferation, increased alkaline phosphatase activity, and enhanced the formation of mineralized nodules in the osteoblast cultures.

Article Abstract

Objective: To investigate the biological effects of the serum of rats fed with kidney-tonifying traditional Chinese drugs on cultured osteoblasts in vitro.

Methods: The culture media containing the serum from rats fed with the drugs at different doses (high, mediate and low doses) were used to treat the second passage of the osteoblasts from the skull of newborn SD rats, and the cell proliferation, differentiation and mineralization were observed.

Results: The serum stimulated the cell proliferation, enhanced alkaline phosphatase activity and increased the number of mineralized nodules in the cultured osteoblasts.

Conclusion: Kidney-tonifying traditional Chinese drugs can stimulate the proliferation, differentiation and maturation of cultured osteoblasts in vitro.

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