Background: A cytomegalovirus-like 'stealth virus' had previously been isolated from a patient with the chronic fatigue syndrome (CFS).
Objective: To determine the original derivation of this virus.
Study Design: DNA sequencing of cloned regions of the virus was performed and the sequences were compared using BLASTN and FASTA analyses against the entire GenBank database. Viral sequences were also used to design primers for the polymerase chain reaction (PCR).
Results: DNA and amino acid sequence comparisons showed that the stealth virus was more closely related to the Colburn strain of simian cytomegalovirus (SCMV) than to CMV of either human or rhesus monkey origin or to any other sequenced herpesvirus. Similarity, but non-identity, between the stealth virus and SCMV, was confirmed using PCR.
Conclusion: The findings implicate the African green monkey as the probable source of the virus isolated from this CFS patient.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0928-0197(95)00020-9 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Decoding Plant-Based Green Synthesis of Zinc Oxide Nanoparticles.
Chem Biodivers
January 2025
Physics Department, Centre National de la Recherche Scientifique (Unité Mixte de Recherche 6283), Institut des Molécules et Matériaux du Mans, Le Mans Université, Le Mans, France.
This study presents, for the first time, the comparison of behavior between two commonly found plant species, their extracts, and their major constituents (glucose and sucrose constituting over 70% of their dried extract) to synthesize zinc oxide (ZnO) nanoparticles (NPs) from zinc nitrate hexahydrate. The findings underscore the critical role of sugars as key constituents in facilitating this synthesis. This research demonstrates that the process can occur at relatively low temperatures (120°C).
View Article and Find Full Text PDFAntiviral activity of an ACE2-Fc fusion protein against SARS-CoV-2 and its variants.
PLoS One
January 2025
Immunology and Immunotherapy Division, Center of Molecular Immunology (CIM), Havana, Cuba.
SARS-CoV-2 has continued spreading around the world in recent years since the initial outbreak in 2019, frequently developing into new variants with greater human infectious capacity. SARS-CoV-2 and its mutants use the angiotensin-converting enzyme 2 (ACE2) as a cellular entry receptor, which has triggered several therapeutic strategies against COVID-19 relying on the use of ACE2 recombinant proteins as decoy receptors. In this work, we propose an ACE2 silent Fc fusion protein (ACE2-hFcLALA) as a candidate therapy against COVID-19.
View Article and Find Full Text PDFVisible-light-induced CO-releasing properties and cytotoxicity of a Ru(II) carbonyl complex containing 2-(pyridin-2-yl)-quinoxaline.
Dalton Trans
January 2025
Department of Chemistry, Faculty of Science, Cairo University, Gamma Street, Giza, Cairo 12613, Egypt.
The photo-induced CO-releasing properties of the dark-stable complex [RuCl(CO)L] (L = 2-(pyridin-2-yl)quinoxaline) were investigated under 468 nm light exposure in the presence and absence of biomolecules such as histidine, calf thymus DNA and hen egg white lysozyme. The CO release kinetics were consistent regardless of the presence of these biomolecules, suggesting that they did not influence the CO release mechanism. The quinoxaline ligand demonstrated exceptional cytotoxicity against human acute monocytic leukemia cells (THP-1), with evidence of potential DNA damage ascertained by comet assay, while it remained non-toxic to normal kidney epithelial cells derived from African green monkey (Vero) cell lines.
View Article and Find Full Text PDFClinical Manifestations.
Alzheimers Dement
December 2024
Brigham and Women's Hospital, Boston, MA, USA.
Background: The impact of knowledge of β-amyloid status on cognitively unimpaired persons' cognitive test performance is unknown.
Method: Cognitively unimpaired adults aged 65-80 with a first-degree relative with AD received a dementia risk estimate and were randomly assigned to disclosure (D+) or non-disclosure (D-) of their β-amyloid PET scan result. At 6 weeks and 6 months post-disclosure, participants completed the ADCS-PACC, a composite of Free and Cued Selective Reminding (free portion), Logical Memory IIa test, Digit-Symbol Substitution, and MMSE.
ATM/ATR-Mediated DNA Damage Response Facilitates SARS-CoV-2 Spike Protein-Induced Syncytium Formation.
J Med Virol
January 2025
Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, State Key Laboratory of Advanced Medical Materials and Devices, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China.
Multinucleated cells are present in lung tissues of patients infected by SARS-CoV-2. Although the spike protein can cause the fusion of infected cells and ACE2-expressing cells to form syncytia and induce damage, how host cell responses to this damage and the role of DNA damage response (DDR) signals in cell fusion are still unclear. Therefore, we investigated the effect of SARS-CoV-2 spike protein on the fusion of homologous and heterologous cells expressing ACE2 in vitro models, focusing on the protein levels of ATR and ATM, the major kinases responding to DNA damage, and their substrates CHK1 and CHK2.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!