This study was designed to evaluate the possible in vivo induction of DNA damage by exposure to radiation in cardiologists. The alkaline comet assay (single cell gel electrophoresis, SCGE), which appears to be a promising tool with which to estimate DNA damage at the single cell level, has been used. The assay was carried out on 30 cardiologists currently employed in a busy cardiac service and 30 healthy unexposed controls. Venous blood samples were obtained from the exposed and control subjects and SCGE was examined in 100 cells graded as undamaged, intermediate, and tailed nuclei. The number of undamaged nuclei was almost the same in control and exposed subjects. The extent of DNA migration (SCGE assay) did not distinguish between the samples in either the nonsmoker exposed or nonsmoker control subjects, which leads one to wonder whether a difference in DNA damage really exists. Previous studies reported increased DNA damage in blood lymphocytes of smokers. In our study, the percentage of damaged cells increased either with the frequency of smoking or exposure to radiation. A statistically significant difference was observed both in smokers and exposed subjects. In conclusion, the elevated grade of DNA damage in cardiologists exposed to radiation indicates a possible genotoxic hazard, therefore, careful measures and full cooperation between cardiologists and radiologists should be undertaken to reduce the exposure to radiation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1536/jhj.45.845 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Ergothioneine, a New Acrolein Scavenger at Elevated Temperature.
J Agric Food Chem
January 2025
Department of Food Science and Technology, School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, 2# Xuelin Road, Nanjing 210023, People's Republic of China.
Acrolein (ACR) present in vivo and in vitro can damage proteins and DNA, linking it to various chronic diseases. In this paper, ergothioneine (EGT), abundant in edible mushrooms, has been studied for its ability to trap ACR and its reaction pathway with ACR at high temperatures using ultraperformance liquid chromatography-mass spectrometry (UPLC-MS/MS). We synthesized the adducts (EGT-ACR-1 and EGT-ACR-2), elucidating their structure and reaction site through HRMS and nuclear magnetic resonance.
View Article and Find Full Text PDFDNA-Dependent Protein Kinase Catalytic Subunit Prevents Ferroptosis in Retinal Pigment Epithelial Cells.
Invest Ophthalmol Vis Sci
January 2025
Department of Ophthalmology of Tongji Hospital and Laboratory of Clinical and Visual Sciences of Tongji Eye Institute, School of Medicine, Tongji University, Shanghai, China.
Purpose: The purpose of this study was to investigate the activated core kinases involved in the DNA damage responses (DDR) during ferroptosis of retinal pigment epithelial (RPE) cells in vitro and their regulatory effects on ferroptosis.
Methods: Ferroptosis was induced by erastin in induced RPE (iRPE) cells derived from human umbilical cord mesenchymal stem cells (hUCMSCs), hUCMSCs, and induced pluripotent stem cell-derived RPE (iPSC-RPE) cells. CCK8 was employed to measure the cell viability.
Colorectal carcinoma (CRC) progression is associated with an increase in PROX1+ tumor cells, which exhibit features of CRC stem cells and contribute to metastasis. Here, we aimed to provide a better understanding to the function of PROX1+ cells in CRC, investigating their progeny and their role in therapy resistance. PROX1+ cells in intestinal adenomas of ApcMin/+ mice expressed intestinal epithelial and CRC stem cell markers, and cells with high PROX1 expression could both self-renew tumor stem/progenitor cells and contribute to differentiated tumor cells.
View Article and Find Full Text PDFWhile key for pathogen immobilization, neutrophil extracellular traps (NETs) often cause severe bystander cell/tissue damage. This was hypothesized to depend on their prolonged presence in the vasculature, leading to cytotoxicity. Imaging of NETs (histones, neutrophil elastase, extracellular DNA) with intravital microscopy in blood vessels of mouse livers in a pathogen-replicative-free environment (endotoxemia) led to detection of NET proteins attached to the endothelium for months despite the early disappearance of extracellular DNA.
View Article and Find Full Text PDFMineral Stress Drives Loss of Heterochromatin: An Early Harbinger of Vascular Inflammaging and Calcification.
Circ Res
January 2025
British Heart Foundation Centre for Research Excellence, School of Cardiovascular and Metabolic Medicine and Sciences, James Black Centre, King's College London, United Kingdom (C.Y.H., M.-Y.W., J.T., S.A., L.D., G.A., R.H., C.M.S.).
Background: Vascular calcification is a detrimental aging pathology markedly accelerated in patients with chronic kidney disease. Prelamin A is a biomarker of vascular smooth muscle cell aging that accelerates calcification however the mechanisms remain undefined.
Methods: Vascular smooth muscle cells were transduced with prelamin A using an adenoviral vector and epigenetic modifications were monitored using immunofluorescence and targeted polymerase chain reaction array.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!