Anthrax is an infectious disease caused by toxigenic strains of the Gram-positive bacterium Bacillus anthracis, which is mainly present in the environment in the form of highly resistant spores. In order to elucidate a surface enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectroscopy analysis to profile different expressed proteins when B. anthracis spores are infected in human macrophages, we analyzed human macrophage cytosolic fractions for the infection of B. anthracis spores. Eleven different protein peaks were obtained. The 8217.8 kDa was increased specifically in inactivated-Sterne spores at 90 min. At 120 min, the peak of 8552.1 kDa in the inactivate-Sterne spores increased more than fourfold compared to live-Sterne spores. The protein peak at 8552.1kDa suggests that inactivated-Sterne spores could cause the phagolysosome formation of macrophages. And the protein peaks that increased in live-Sterne spores suggest that it could escape from the phagolysosome of the macrophage. These SELDI-TOF profiles assume an important role in human macrophage for the survival and escape of the infected B. anthracis spores.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.bbrc.2004.10.146 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Toxin and capsule production by Bacillus cereus biovar anthracis influence pathogenicity in macrophages and animal models.
PLoS Negl Trop Dis
December 2024
Emerging Pathogens Institute, University of Florida, Gainesville, Florida, United States of America.
Bacillus cereus biovar anthracis (Bcbva) causes anthrax-like disease in animals, particularly in the non-human primates and great apes of West and Central Africa. Genomic analyses revealed Bcbva as a member of the B. cereus species that carries two plasmids, pBCXO1 and pBCXO2, which have high sequence homology to the B.
View Article and Find Full Text PDFFrom Spores to Suffering: Understanding the Role of Anthrax in Bioterrorism.
Mil Med
December 2024
Division of Clinical Research and Medical Management (CRMM), Institute of Nuclear Medicine & Allied Sciences (INMAS), DRDO, Delhi 110054, India.
Introduction: Anthrax, caused by the bacterium Bacillus anthracis, stands as a formidable threat with both natural and bioterrorism-related implications. Its ability to afflict a wide range of hosts, including humans and animals, coupled with its potential use as a bioweapon, underscores the critical importance of understanding and advancing our capabilities to combat this infectious disease. In this context, exploring futuristic approaches becomes imperative, as they hold the promise of not only addressing current challenges but also ushering in a new era in anthrax management.
View Article and Find Full Text PDFArchitecture of the Sap S-layer of revealed by integrative structural biology.
Proc Natl Acad Sci U S A
December 2024
Structural and Molecular Microbiology, Vlaams Instituut voor Biotechnologie (VIB)-Vrije Universiteit Brussel (VUB) Center for Structural Biology, Vlaams Instituut voor Biotechnologie, Brussels 1050, Belgium.
is a spore-forming gram-positive bacterium responsible for anthrax, an infectious disease with a high mortality rate and a target of concern due to bioterrorism and long-term site contamination. The entire surface of vegetative cells in exponential or stationary growth phase is covered in proteinaceous arrays called S-layers, composed of Sap or EA1 protein, respectively. The Sap S-layer represents an important virulence factor and cell envelope support structure whose paracrystalline nature is essential for its function.
View Article and Find Full Text PDFClosing the Gaps: Testing the Efficacy of Carbapenem and Cephalosporin Treatments of Late-Stage Anthrax in Rabbits.
Pathogens
October 2024
Department of Infectious Diseases, Israel Institute for Biological Research, Ness-Ziona P.O. Box 19, Israel.
Anthrax is a fatal zoonotic disease caused by exposure to spores. The CDC's guidelines divide anthrax treatment into three categories according to disease progression: post-exposure prophylaxis (PEP), systemic, and systemic with a suspicion of CNS infection. While the prognosis for PEP or the early treatment of systemic anthrax is very good, ingress of the bacteria into the CNS poses a substantial clinical challenge.
View Article and Find Full Text PDFComparing microbiological and molecular diagnostic tools for the surveillance of anthrax.
PLoS Negl Trop Dis
November 2024
Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.
The diagnosis of anthrax, a zoonotic disease caused by Bacillus anthracis can be complicated by detection of closely related species. Conventional diagnosis of anthrax involves microscopy, culture identification of bacterial colonies and molecular detection. Genetic markers used are often virulence gene targets such as B.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!