Antibiotic activity screening of 15 species of Aspergillus parvulus were made relative to different test-organisms (gram positive, gram negative, phytopathogenic bacteria and yeasts) and phytotoxic activity screening relative to different algae. The researched strains 3387, 340, 3142, 1813 of A. parvulus had a wide spectrum of antibiotic activity and high phytotoxic activity. The rest of species had different degrees of antibiotic and phytotoxic activity. For further investigation the evaporated cultural filtrate of A. parvulus 3142 was separated by the method of column chromatography into fractions with different properties. Minimum suppressing concentration (which equals 2 mg/ml for most active fraction) was determined for the most active one, and a system of solvents was chosen as a results of Schevchic assembly chromatography for the extraction of active substance (n-buthanol saturated water).
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