In this study, the behavior of bone marrow stromal cells cultured on calcium titanium phosphate (CTP) microspheres was analyzed. Cell adhesion and proliferation were estimated by the neutral red assay and by total DNA quantification. Morphology and deposition of extracellular matrix were assessed by confocal laser scanning microscopy and/or scanning electron microscopy. The expression of the osteoblastic phenotype was evaluated by monitoring alkaline phosphatase activity and osteocalcin secretion. Results revealed that cells were able to attach and spread on the surface of CTP microspheres, and gradually grow into nearly confluent monolayers. Moreover, cells were able to bridge adjacent microspheres forming microsphere-cell clusters. Cells produced an abundant amount of fibrillar extracellular matrix that covered the substrate surface. Alkaline phosphatase activity peaked around days 7-14 and then decreased until day 21. Cells secreted osteocalcin, with higher levels being detected at day 14 than at day 21. Taken together, these results suggest that CTP microspheres are appropriate scaffolds for the growth and differentiation of cells along the osteoblastic lineage.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/jbm.a.30217 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Approaching high-performance lithium storage materials of CoNiO microspheres wrapped coal tar pitch-derived porous carbon.
Dalton Trans
June 2023
School of Materials Science and Engineering, Northeastern University, Shenyang 110819, PR China.
Ternary transition metal oxides (TMOs) are deemed as promising anode materials of Li-ion batteries (LIBs) owing to their large theoretical capacity and rich redox reaction. Nevertheless, the inherent semiconductor characteristic and enormous volume variation of TMOs during cycling bring about sluggish reaction kinetics, fast capacity fading, and poor rate capability. In this study, three-dimensional (3D) porous CoNiO@CTP architectures, , CoNiO microspheres combined with coal tar pitch-derived porous carbon, were designed and synthesized through a one-step hydrothermal method followed by a heat treatment process for the first time.
View Article and Find Full Text PDFChiral covalent organic framework core-shell composite CTpBD@SiO used as stationary phase for HPLC enantioseparation.
Mikrochim Acta
August 2021
Department of Chemistry, Yunnan Normal University, Kunming, 650500, People's Republic of China.
The fascinating framework structures and unique properties of chiral covalent organic frameworks (COFs) make them promising candidates as novel separation medium for high-performance liquid chromatography (HPLC). However, the irregular morphology, inhomogeneous particle size, and low density of conventional COF particles will lead to a low column efficiency, undesirable chromatographic peak shape, and high column backpressure of such COF-packed columns. In this work, a chiral COF CTpBD was synthesized by the Schiff base reaction between benzidine (BD) and chiral organic monomer CTp obtained through the reaction of 1,3,5-triformylphoroglucinol (Tp) and (+)-diacetyl-L-tartaric anhydride ((+)-Ac-L-Ta).
View Article and Find Full Text PDFAssessment of Semi-automated Computed Tomographic Measures of Segmental Perfusion Defects in a Swine Model () of Intermediate Coronary Lesions.
Comp Med
June 2020
Cardiology Service, Brooke Army Medical Center, Fort Sam Houston, Texas;, Email:
Computed tomographic myocardial perfusion (CTP) imaging is a tool that shows promise in emergent settings for defining the hemodynamic significance of coronary artery disease. In this study, we examined the accuracy with which the transmural perfusion ratio (TPR) derived through semiautomated CTP analysis reflected segmental perfusion defects associated with intermediate coronary artery lesions in swine. Lesions (diameter stenosis, 65% ± 11%) of the left anterior descending coronary artery (LAD) were created in 10 anesthetized female swine (weight, 47.
View Article and Find Full Text PDFControlled Fabrication of Silica@Covalent Triazine Polymer Core-Shell Spheres as a Reversed-Phase/Hydrophilic Interaction Mixed-Mode Chromatographic Stationary Phase.
ACS Appl Mater Interfaces
December 2019
College of Chemistry and Molecular Engineering , Zhengzhou University, Zhengzhou 450001 , People's Republic of China.
The unique properties of covalent triazine-based organic framework/polymers, including large surface area, hydrophilic-lipophilic-balanced adsorption, and economical preparation, make it a promising candidate as a stationary phase for high-performance liquid chromatography. However, irregular shapes and wide size distributions of such particles hinder column packing, resulting in a low column efficiency or a high back pressure. Herein, we describe the fabrication of SiO@ covalent triazine-based organic polymer (CTP) core-shell microspheres with a distinct sphere-coating-sphere appearance using aminosilica as the supporting substrate to grow the CTP shell.
View Article and Find Full Text PDFRoom temperature synthesis and binding studies of solution-processable histamine-imprinted microspheres.
J Mol Recognit
January 2018
Department of Chemistry, Ateneo de Manila University, Quezon City, Philippines.
Accurate quantification of histamine levels in food and in biological samples is important for monitoring the quality of food products and for the detection of pathophysiological conditions. In this study, solution processable histamine-imprinted microspheres were synthesized at 30°C via dilute free radical phototochemical polymerization technique using ethylene glycol dimethacrylate (EGDMA) as the crosslinker and methacrylic acid (MAA) as the monomer. The processability of the resulting polymer is dictated by the monomer feed concentration (eg, 4 wt% 80:20 EGDMA:MAA formulation) and solvent (acetonitrile).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!