AI Article Synopsis
- Pinin/DRS (Pnn) is a phosphoprotein that plays a role in cell adhesion and the activity of tumor suppressor genes, primarily located in nuclear speckles for mRNA processing.
- Pnn has been shown to interact with the transcriptional corepressor CtBP1, which affects E-cadherin promoter activity.
- This interaction implies that Pnn may influence transcriptional repression mechanisms and could connect mRNA processing with transcription regulation.
Article Abstract
Previously, we have shown that pinin/DRS (Pnn), a 140-kDa nuclear and cell adhesion-related phosphoprotein, is involved in the regulation of cell adhesion and modulation of the activity of multiple tumor suppressor genes. In the nucleus Pnn is concentrated in the "nuclear speckles," zones of accumulation of transcriptional and mRNA splicing factors, where Pnn is involved in mRNA processing. Alternatively, other roles of Pnn in gene regulation have not yet been established. By utilizing in vitro pull-down assays, in vivo interaction studies, and immunofluorescence in combination with overexpression and RNA interference experiments, we present evidence that Pnn interacts with the known transcriptional corepressor CtBP1. As a consequence of this interaction Pnn was capable of relieving the CtBP1-mediated repression of E-cadherin promoter activity. Our results suggest that the interaction of Pnn with the corepressor CtBP1 may modulate repression of transcription by CtBP1. This interaction may reflect the existence of coupling factors involved in CtBP-mediated transcriptional regulation and mRNA processing events.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC529029 | PMC |
| http://dx.doi.org/10.1128/MCB.24.23.10223-10235.2004 | DOI Listing |
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