Reetha (Sapindus trifoliata) seed extract and Chitosan were used as additives in the sludge bed of a UASB reactor treating low strength wastewater to enhance granulation. Five parallel laboratory scale UASB reactors were operated for 250 days with synthetic wastewater feed containing COD in the range of 600-800 mg/L. The reactors were seeded with spent sludge from a full-scale 5MLD UASB treatment plant at Jajmau, Kanpur, India. The seed sludge contained little or no granules. Different additives in the five reactors were as follows: control with no additive, cationic part of Reetha extract as additive, anionic part of Reetha extract as additive, bulk Reetha extract as additive and Chitosan as additive. The granulation rapidly increased in all the reactors beyond the 90th day of operation. The mean granule sizes as well as the fraction of granular sludge (particle size > or = 100 microm) were more in the presence of some of the additives compared to the control reactor. Chitosan significantly enhanced granulation followed by the cationic and anionic fractions of the Reetha extract. The bulk Reetha extract did not show enhancement of granulation. The ESEM/EDAX results showed that the bigger granules (3-4 mm) had porous structure and appeared as conglomerates of smaller granules.
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