Colonization of Phlebotomus neglectus Tonnoir, the major vector of visceral leishmaniasis, in Greece is reported for the first time. Starting with wild-caught specimens, a small closed colony was established that was maintained for 17 mo or 10 generations. Gonotrophic discordance, stenogamic mating behavior, low fecundity, and dormancy because of low temperature were the most important findings that characterized the colony.
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http://dx.doi.org/10.1093/jmedent/37.3.346 | DOI Listing |
Proc Natl Acad Sci U S A
December 2024
Intracellular Parasite Biology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892.
Sandfly vectors transmit through egestion of parasites into the host skin. The transmissible dose is shaped by development in the sandfly gut, described as a sequential differentiation of promastigote morphotypes. Apart from isolated mammal-infective metacyclic promastigotes, little is known about the transcriptional programs and molecular markers for other stages coinhabiting the midgut in mature infections and cotransmitted by the sandfly bite.
View Article and Find Full Text PDFPLoS Negl Trop Dis
October 2024
Department of Parasitology, Faculty of Science, Charles University, Prague, Czechia.
Background: Several new species of Leishmania have recently emerged in Europe, probably as the result of global changes and increased human migration from endemic areas. In this study, we tested whether two sand fly species, the Western Mediterranean Phlebotomus perniciosus and the Eastern Mediterranean P. tobbi, are competent vectors of L.
View Article and Find Full Text PDFInt J Parasitol Drugs Drug Resist
August 2024
UMR 8076 BioCIS, CNRS, Université Paris-Saclay, 91400, Orsay, France; UMR BIPAR, Laboratory of Animal Health, Anses, INRAe, EnvA, 94700, Maisons-Alfort, France; National Malaria Reference Center, AP-HP, Hôpital Bichat Claude Bernard, 75018, Paris, France.
Leishmania major is responsible for zoonotic cutaneous leishmaniasis. Therapy is mainly based on the use of antimony-based drugs; however, treatment failures and illness relapses were reported. Although studies were developed to understand mechanisms of drug resistance, the interactions of resistant parasites with their reservoir hosts and vectors remain poorly understood.
View Article and Find Full Text PDFParasit Vectors
January 2024
Department of Microbiology, Immunology, and Infectious Diseases, Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada.
Background: Vector sand fly colonies are a critical component of studies aimed at improving the understanding of the neglected tropical disease leishmaniasis and alleviating its global impact. However, among laboratory-colonized arthropod vectors of infectious diseases, the labor-intensive nature of sand fly rearing coupled with the low number of colonies worldwide has generally discouraged the widespread use of sand flies in laboratory settings. Among the different factors associated with the low productivity of sand fly colonies, mite infestations are a significant factor.
View Article and Find Full Text PDFmBio
December 2022
Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
Genetic exchange between different strains in the sand fly vector has been experimentally demonstrated and is supported by population genetic studies. In nature, opportunities for interstrain mating are restricted to flies biting multiply infected hosts or through multiple bites of different hosts. In contrast, self-mating could occur in any infected sand fly.
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