A L-rhamnose transport-negative strain of Escherichia coli was generated by Mu d(ApR,lac)I mutagenesis. This strain was used to isolate a clone of Salmonella typhimurium DNA that encoded L-rhamnose-H+ transport activity, the gene for which, rhaT, was sequenced. The rhaT gene was mapped on the E. coli chromosome between rhaR and sodA at 87.9 min, initially by Southern blot analysis and then by the isolation, expression, and sequencing of the rhaT gene. Both rhaT genes encoded a hydrophobic protein of 344 amino acids (91% identical) that contained 10 putative transmembrane regions. The RhaT protein represents a novel class of sugar transport protein.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Transcriptome profiles of Trypanosoma brucei rhodesiense in Malawi reveal focus specific gene expression profiles associated with pathology.
PLoS Negl Trop Dis
May 2024
Department of Biochemistry and Sports Sciences, College of Natural Sciences, Makerere University, Kampala, Uganda.
Background: Sleeping sickness caused by Trypanosoma brucei rhodesiense is a fatal disease and endemic in Southern and Eastern Africa. There is an urgent need to develop novel diagnostic and control tools to achieve elimination of rhodesiense sleeping sickness which might be achieved through a better understanding of trypanosome gene expression and genetics using endemic isolates. Here, we describe transcriptome profiles and population structure of endemic T.
View Article and Find Full Text PDFDifferences in gene expression profiles in early and late stage rhodesiense HAT individuals in Malawi.
PLoS Negl Trop Dis
December 2023
Kamuzu University of Health Sciences, Malawi-Liverpool-Wellcome Trust Clinical Research Programme, Blantyre, Malawi.
T. b. rhodesiense is the causative agent of Rhodesian human African trypanosomiasis (r-HAT) in Malawi.
View Article and Find Full Text PDFSHERLOCK4HAT: A CRISPR-based tool kit for diagnosis of Human African Trypanosomiasis.
EBioMedicine
November 2022
Trypanosome Molecular Biology, Department of Parasites and Insect Vectors, Institut Pasteur, Université Paris Cité, F-75015, Paris, France. Electronic address:
Background: To achieve elimination of Human African Trypanosomiasis (HAT) caused by Trypanosoma brucei gambiense (gHAT), the development of highly sensitive diagnostics is needed. We have developed a CRISPR based diagnostic for HAT using SHERLOCK (Specific High-sensitivity Enzymatic Reporter unLOCKing) that is readily adaptable to a field-based setting.
Methods: We adapted SHERLOCK for the detection of T.
The l-rhamnose-dependent regulator RhaS and its target promoters from expand the genetic toolkit for regulatable gene expression in the acetic acid bacterium .
Front Microbiol
August 2022
Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Jülich, Germany.
For regulatable target gene expression in the acetic acid bacterium (AAB) only recently the first plasmids became available. These systems solely enable AraC- and TetR-dependent induction. In this study we showed that the l-rhamnose-dependent regulator RhaS from and its target promoters P , P , and P could also be used in for regulatable target gene expression.
View Article and Find Full Text PDFRhamnosyltransferases involved in the biosynthesis of flavone rutinosides in Chrysanthemum species.
Plant Physiol
November 2022
Research Center of Chinese Herbal Resource Science and Engineering, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
Linarin (acacetin-7-O-rutinoside), isorhoifolin (apigenin-7-O-rutinoside), and diosmin (diosmetin-7-O-rutinoside) are chemically and structurally similar flavone rutinoside (FR) compounds found in Chrysanthemum L. (Anthemideae, Asteraceae) plants. However, their biosynthetic pathways remain largely unknown.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!