AI Article Synopsis
- A new transfected cell array (TCA) technology enables the simultaneous transfection of thousands of DNA molecules in a microarray format, allowing researchers to study the effects of overexpressing many proteins in one experiment.
- The TCA technique is also useful for RNA interference (RNAi) research, where small interfering RNAs (siRNA) and plasmids can be reverse-transfected into cells, facilitating gene silencing analysis.
- This review highlights the synergy between cell arrays and RNAi for conducting high-throughput loss-of-function studies, providing insights into gene function and cellular responses.
Article Abstract
A recently established transfected cell array (TCA) technology has opened new experimental dimensions in the field of functional genomics. Cell arrays allow for transfection of several thousands different DNA molecules in microarray format. The effects of overexpression of hundreds of proteins on cellular physiology can be observed in a single experiment. The TCA technique has also found its application in RNA interference (RNAi) research. Small interfering RNAs (siRNA) as well as plasmid expressing short hairpin RNAs can be transferred into the cells through the process of reverse transfection. The silencing of numerous genes in spatially separated manner can be thus monitored. This review will provide an overview on current concepts concerning combination of cell array and RNAi for high-throughput loss-of-function studies.
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| http://dx.doi.org/10.1038/sj.onc.1208027 | DOI Listing |
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