The biochemical character of PCD in different interaction between wheat and Puccinia recondita f. sp tritici was observed using DNA agarose gel electrophoresis and end labeling in situ (Terminal Deoxynucleotidyl Transferase-mediated dUTP Nick End TUNEL). The DNA ladder, which is the typical character of programmed cell death(PCD), was observed in incompatible combination (cv.lorvin10 x race 162) and the positive reaction in wheat leaves of incompatible combination since 12h after inoculation also appeared. The results, coupled with the previous study, indicated that HR was a typical form of PCD in incompatible combination between wheat and wheat leaf fungus.
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