In this work, SPLITT Fractionation (split flow thin cell) is used to sort hydrodynamically sedimented particles coming from the Sacca di Goro, a lagoon-like system close to the Po River delta (Italy). First the possibility of performing quantitative mass separations with a SPLITT cell apparatus was checked on a standard silica sample of known particle size distribution (PSD). Environmental sediment samples and relative SPLITT sub-fractions were subject to Inductive Coupled Plasma--Atomic Emission Spectroscopy (ICP-AES) characterization for the following elements: Al, Fe, Cd, Cr, Cu, Ni, Mn, Pb and Zn. The distribution of these metals by particle size fractions has been investigated. The accuracy of the entire separation procedure has been also evaluated.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/adic.200490079 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Experiment, theory, and simulation of a flow-electrical-split flow thin particle separation device.
J Chromatogr A
December 2021
Department of Mechanical Engineering, University of Utah, Salt Lake, UT 84112, USA.
Herein, we describe the simulation of a novel flow-electrical-split flow thin (Fl-El-SPLITT) separation device and validate it using existing theory and experimentation for the first time using polystyrene particles of 28 and 1000 nm diameters. The fraction of particles exiting selected ports with DC El-SPLITT is predicted with existing theory, but the theory does not include AC fields, nor does it incorporate the use of crossflows. Using DC fields the El-SPLITT simulation and theory calculated transition points result in the same values.
View Article and Find Full Text PDFDevelopment and Testing of a Continuous Flow-Electrical-Split-Flow Lateral Transport Thin Separation System (Fl-El-SPLITT).
Anal Chem
February 2021
Department of Mechanical Engineering, University of Utah, 1495 E 100 S, Salt Lake City, Utah 84112, United States.
In this work, a new high-volume, continuous particle separation device that separates based upon size and charge is described. Two continuous flow-electrical-split-flow lateral transport thin (Fl-El-SPLITT) device architectures (a platinum electrode on a porous membrane and a porous graphite electrode under a membrane) were developed and shown to improve particle separations over a purely electrical-SPLITT device. The graphite FL-El-SPLITT device architecture achieved the best separation of approximately 60% of small (28 nm) vs large (1000 nm) polystyrene particles.
View Article and Find Full Text PDFRare earth elements in fine fraction (<20 μm) of the Vistula River sediments.
Chemosphere
December 2019
Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University Gagarina 7, 87-100 Toruń, Poland.
Present studies facilitated testing the hypothesis that sediment fraction <20 μm contains different amounts of REEs. The potentially bioavailable rare earth elements (REEs) were determined in the following fractions of the surface sediments of the Vistula River: <5 μm, 5-10 μm, 10-20 μm, and total <20 μm. The attempt was made to fractionate the sediment by self-adjustable split-flow transport-thin fractionation channel operating in the full depletion mode (SPLITT-FFD-SA), which is a new concept and so far unused method for such purposes.
View Article and Find Full Text PDFMorphological and electrical disturbances after split-flow fractionation in murine macrophages.
J Chromatogr A
April 2019
Centro Internacional de Física (CIF), Laboratorio de Biofísica, Bogotá DC, Colombia; Universidad Nacional de Colombia, Department of Biology, Bogotá DC, Colombia.
Split-flow fractionation (SPLITT) is a family of techniques that separates in the absence of labeling using very low flow rates and force fields, and is therefore expected to minimize cell damage. Although it has been documented that separation methods cause physiological changes in immune cells that are attributable to mechanical stress and antibody labeling, SPLITT has not yet been examined for possible damaging effects of hydrodynamic stress, partly because it is assumed that the low flow rates and weak forces used in this technique do not generate significant mechanical stress. The aim of this study was to investigate the effects of SPLITT on cell function of a murine macrophage cell, and to compare these effects with those induced by centrifugation.
View Article and Find Full Text PDFQuantifying the contribution of recessive coding variation to developmental disorders.
Science
December 2018
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, UK.
We estimated the genome-wide contribution of recessive coding variation in 6040 families from the Deciphering Developmental Disorders study. The proportion of cases attributable to recessive coding variants was 3.6% in patients of European ancestry, compared with 50% explained by de novo coding mutations.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!