Despite evidence that protein kinases are regulators of apoptosis, a specific role for phosphatases in regulating cell survival has not been established. Here we show that alpha4, a noncatalytic subunit of protein phosphatase 2A (PP2A), is required to repress apoptosis in murine cells. alpha4 is a nonredundant regulator of the dephosphorylation of the transcription factors c-Jun and p53. As a result of alpha4 deletion, multiple proapoptotic genes were transcribed. Either inhibition of new protein synthesis or Bcl-xL overexpression suppressed apoptosis initiated by alpha4 deletion. Thus, mammalian cell viability depends on repression of transcription-initiated apoptosis mediated by a component of PP2A.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1126/science.1100537 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Efficient gene deletion of Integrin alpha 4 in primary mouse CD4 T cells using CRISPR RNA pair-mediated fragmentation.
Front Immunol
December 2024
Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
The functional specialization of CD4 T lymphocytes into various subtypes, including T1 and T cells, is crucial for effective immune responses. T cells facilitate B cell differentiation within germinal centers, while T1 cells are vital for cell-mediated immunity against intracellular pathogens. Integrin α4, a cell surface adhesion molecule, plays significant roles in cell migration and co-stimulatory signaling.
View Article and Find Full Text PDFGenetic screening of α-thalassemia fusion gene using routine flow-through hybridization.
Front Genet
November 2024
Center for Medical Genetics and Prenatal Diagnosis, People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi, China.
Article Synopsis
- The study explores the detection of a rare fusion gene associated with α-thalassemia, which can be mistakenly identified as normal or a different mutation using standard testing methods.
- Over a sample period from January 2019 to January 2024, 32,083 samples were tested, revealing that 25 samples (0.08%) initially appeared suspicious for a common -α deletion but were ultimately found to be negative upon further testing.
- The research concludes that while conventional methods can lead to misdiagnosis, flow-through hybridization is an effective way to screen for the fusion gene, which was identified in a small percentage of the Guangzhou population.
Phenotypic Analysis of the : C.95 G > A Mutation in China.
Hemoglobin
September 2024
Precision Medical Lab Center, People's Hospital of Yangjiang, Yangjiang, Guangdong, People's Republic of China.
This study aimed to analyze the clinical phenotype of the : c.95G>A mutation in the Chinese population and to provide guidance for clinical diagnosis and genetic counseling. Peripheral blood samples were collected from 16 patients, including 6 newborns, 2 children, and 8 adults.
View Article and Find Full Text PDFDevelopment and clinical validation of a novel detection kit for α-thalassemia in southern Chinese.
Front Genet
September 2024
Precision Medical Lab Center, People's Hospital of Yangjiang, Yangjiang, Guangdong Province, China.
Article Synopsis
- The study developed a new reverse dot blot assay for detecting 10 types of α-thalassemia alleles prevalent in the Chinese population, focusing on both common and rare variants.
- It involved using a multiplex PCR system to analyze genomic DNA samples from three hospitals, with a total of 1,148 participants, including thalassemia patients and healthy controls.
- The results showed 100% accuracy in genotyping the alleles, demonstrating the assay's effectiveness for genetic screening and clinical diagnosis of α-thalassemia.
SNPscan Combined With CNVplex as a High-Performance Diagnostic Method for Thalassemia.
Prenat Diagn
October 2024
Department of Medical Genetics, School of Basic Medicine Science, Southern Medical University, Guangdong, China.
Objective: Thalassemia is a Mendelian-inherited blood disorder with severe consequences, including disability and mortality, making it a significant public health concern. Therefore, there is an urgent need for precise diagnostic technologies. We introduce two innovative diagnostic techniques for thalassemia, SNPscan and CNVplex, designed to enhance molecular diagnostics of thalassemia.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!