The ratio of purple line: no-purple line(13:3) was observed in six different F2 populations produced by crossing between parents with purple line and no-purple line in coleoptile. The backcross of XNA//XNA/ 21A150 (XNA, no-purple line and CMS, as the recurrent parent) resulted in a ratio of 1:1 (purple line: no-purple line). Genetic analysis showed that the expression of rice coleoptile purple line was influenced by two genes, inhibiting gene I and anti-inhibiting gene Ai(t). I gene inhibits P gene of C_A_P_ system and Ai(t) inhibits / gene, respectively. The gene pools of Ai(t) ai(t) and ai(t) ai(t) were constructed with BF1 of XNA//XNA/21A150. SSR analysis indicated that Ai(t) gene was linked with the markers of RM335, RM295, RM287 and RM21 and the genetic distance from Ai(t) to these four markers were 2.8 cM, 10.2 cM, 13.9 cM, 26.1 cM, respectively.
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