The principles of modern forensic DNA-analysis using the amplification of so called STR-polymorphisms are explained. Four cases from practise that required the collaboration of forensic molecular biology and pathology and which were analysed by them are presented and discussed. Similarities and variations in the work of both "disciplines" are presented and suggestions for the storage of samples formulated.
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http://dx.doi.org/10.1007/s00292-004-0725-2 | DOI Listing |
Forensic Sci Int
January 2025
Center for Medical Ethics and Health Policy, Baylor College of Medicine, Houston, TX, USA.
In 2018, after law enforcement announced it had used a technique called forensic investigative genetic genealogy (FIGG) to identify the Golden State Killer, we conducted a U.S. general population survey and found most respondents supported using FIGG to solve violent crimes.
View Article and Find Full Text PDFSci Rep
January 2025
The Biology and DNA Section, General Department of Forensic Science and Criminology, Dubai Police General Head Quarters, Dubai, United Arab Emirates.
This study evaluated the effectiveness of the amplicon RX post-PCR clean-up kit in enhancing trace DNA profile recovery from forensic casework samples amplified using the GlobalFiler PCR amplification kit. The impact of post-PCR clean-up on allele recovery and signal intensity was assessed in both trace casework samples and control samples across a range of DNA concentrations. The results showed that the amplicon RX method significantly improved allele recovery compared to the 29-cycle protocol (p = 8.
View Article and Find Full Text PDFMethods Protoc
January 2025
The Center for Forensic Science Research and Education, 206 Welsh Road, Horsham, PA 19440, USA.
This differential extraction protocol details the steps for isolating DNA from sample pads used in lateral flow immunochromatographic (LFI) tests, particularly for cases involving mixed biological samples such as semen and menstrual blood, or other evidence related to sexual assault. This procedure utilizes a differential extraction technique applied to sample pads from immunochromatographic tests, where the sample pads serve as the substrate. The method involves two sequential lysis steps to effectively separate non-sperm and sperm fractions, enabling the targeted isolation of distinct cell types for downstream DNA analysis.
View Article and Find Full Text PDFInt J Legal Med
January 2025
Biological and Life Sciences, School of Arts and Sciences, Ahmedabad University, Central Campus, Navrangpura, Ahmedabad, Gujarat, India.
Polymerase Chain Reaction (PCR) has transformed forensic DNA analysis but is still limited when dealing with compromised trace or inhibitor-containing samples. Nanotechnology has been integrated into nanoPCR (nanoparticle-assisted PCR) to overcome these obstacles. Nanomaterials improve PCR sensitivity, selectivity, and efficiency.
View Article and Find Full Text PDFSci Rep
January 2025
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.
Technological advancements have allowed the detection of increasingly complex forensic genetics samples, as minimum amounts of DNA can now be detected in crime scenes or other settings of interest. The weight of the evidence depends on several parameters regarding the population and sample-related analytical factors, the latter in a greater number when the DNA amount is considered. This led to the development of probabilistic genotyping software (PGS), able to deal with the associated complexities.
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