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Unveiling the potential of bean proteins: Extraction methods, functional and structural properties, modification techniques, physiological benefits, and diverse food applications.
Int J Biol Macromol
January 2025
Centre of Excellence for Postharvest Biotechnology (CEPB), School of Biosciences, University of Nottingham Malaysia, Jalan Broga, Semenyih, Selangor Darul Ehsan 43500, Malaysia; Future Food Beacon of Excellence, Faculty of Science, University of Nottingham, Loughborough LE 12 5RD, United Kingdom.
Bean proteins, known for their sustainability, versatility, and high nutritional value, represent a valuable yet underutilized resource, receiving less industrial attention compared to soy and pea proteins. This review examines the structural and molecular characteristics, functional properties, amino acid composition, nutritional value, antinutritional factors, and digestibility of bean proteins. Their applications in various food systems, including baked goods, juice and milk substitutes, meat alternatives, edible coatings, and 3D printing inks, are discussed.
View Article and Find Full Text PDFTailed molecular beacon probes: an approach for the detection of structured DNA and RNA analytes.
Chem Commun (Camb)
January 2025
Chemistry Department, University of Central Florida, Orlando, Florida 32816, USA.
Molecular beacon (MB) probes have been extensively used for nucleic acid analysis. However, MB probes fail to hybridize with folded DNA or RNA. Here, we demonstrate that MB probes equipped with extra sequences complementary to the analyte, named 'tail', can increase the signal-to-background ratio by ∼40-fold and hybridization rates by ∼800-fold compared to conventional MB probes.
View Article and Find Full Text PDFExogenously and Endogenously Sequential Regulation of DNA Nanodevices Enables Organelle-Specific Signal Amplification in Subcellular ATP Profiling.
Angew Chem Int Ed Engl
January 2025
School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
Adenosine triphosphate (ATP), the primary energy currency in cells, is dynamically regulated across different subcellular compartments. The ATP interplay between mitochondria and endoplasmic reticulum (ER) underscores their coordinated roles in various biochemical processes, highlighting the necessity for precise profiling of subcellular ATP dynamics. Here we present an exogenously and endogenously dual-regulated DNA nanodevice for spatiotemporally selective, subcellular-compartment specific signal amplification in ATP sensing.
View Article and Find Full Text PDFDNAzyme-mediated isothermal catalytic hairpin assembly for rapid and enzyme-free amplified detection of lead(Ⅱ) ion.
J Hazard Mater
January 2025
Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai Ocean University, Shanghai 201306, China; Laboratory of Quality and Safety Risk Assessment for Aquatic Product on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China; Shanghai Ocean University, Shanghai 201306, China. Electronic address:
The detection of heavy metal ions, particularly lead (Pb²⁺), in environmental samples is crucial for public health and safety. Current nucleic acid signal amplification methods for Pb²⁺ detection often rely on biological enzymes and are limited in applicability due to high costs, prolonged detection times, and nonspecific adsorption. In this study, we introduce an enzyme-free, DNAzyme-mediated isothermal catalytic hairpin assembly (DMICHA) assay, which combines a DNAzyme-based Pb²⁺ recognition module with a signal amplification process utilizing isothermal catalytic hairpin assembly (CHA).
View Article and Find Full Text PDFFrom signal-off to signal-on: polyT linker alters signal response mode and enhances signal change of aptamer beacon probe.
Anal Bioanal Chem
January 2025
College of Chemistry and Chemical Engineering, Linyi University, Linyi, 276000, China.
A molecular beacon is an oligonucleotide hybridization probe that can report the presence of specific nucleic acids in homogeneous solutions. Using an aptamer has allowed an aptamer-based molecular beacon-aptamer beacon to be developed, which has shown advantages of simplicity, rapidity, and sensitivity in imaging and sensing non-nucleic acid substances. However, due to requirement for a deliberate DNA hairpin structure for the preparation of a molecular beacon, not any given aptamer is suitable for designing an aptamer beacon probe.
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