Objective: To examine matrix metalloproteinase-3 (MMP-3) expression in human stromal cell culture after P stimulation and the effect of conditioned medium from human embryo-epithelial cells coculture on its expression and activity.
Design: Metabolic and endocrine studies on human tissue.
Setting: In vitro fertilization (i.v.f.) unit and endocrine research unit.
Patient(s): Infertile patients undergoing endometrial tissue sampling for dating at the luteal phase before i.v.f.
Intervention(s): Endometrial sampling and collection of human embryos culture media.
Main Outcome Measure(s): Expression and activity of secreted MMP-3 by P-induced stromal cells, and in stromal cells exposed to conditioned medium from embryo-epithelial cell coculture.
Result(s): Expression and activity of MMP-3 in human stromal cells decrease after P induction. Following incubation of these stromal-derived decidual cells with conditioned medium from embryo-epithelial cell coculture, MMP-3 expression and activity increased in a statistically significant manner.
Conclusion(s): Progesterone inhibition of MMP-3 expression and its support of endometrial integrity were prevented by local expression of MMP-3 in response to embryonic signaling.
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| http://dx.doi.org/10.1016/j.fertnstert.2004.06.026 | DOI Listing |
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