AI Article Synopsis
- The study focuses on understanding the invasion process in cancer metastasis, aiming to identify key proteins involved in tumor cell migration.
- Researchers developed a proteomic screening technique that combines Fluorophore Assisted Light Inactivation (FALI) with an antibody library to systematically test surface proteins on fibrosarcoma cells for their role in invasion.
- Findings reveal that the protein CD155 (poliovirus receptor) plays a significant role in tumor cell migration, as its knockdown leads to reduced migration in fibrosarcoma and glioblastoma cells, indicating its potential contribution to cancer progression.
Article Abstract
Background: Invasion is an important early step of cancer metastasis that is not well understood. Developing therapeutics to limit metastasis requires the identification and validation of candidate proteins necessary for invasion and migration.
Methods: We developed a functional proteomic screen to identify mediators of tumor cell invasion. This screen couples Fluorophore Assisted Light Inactivation (FALI) to a scFv antibody library to systematically inactivate surface proteins expressed by human fibrosarcoma cells followed by a high-throughput assessment of transwell invasion.
Results: Using this screen, we have identified CD155 (the poliovirus receptor) as a mediator of tumor cell invasion through its role in migration. Knockdown of CD155 by FALI or by RNAi resulted in a significant decrease in transwell migration of HT1080 fibrosarcoma cells towards a serum chemoattractant. CD155 was found to be highly expressed in multiple cancer cell lines and primary tumors including glioblastoma (GBM). Knockdown of CD155 also decreased migration of U87MG GBM cells. CD155 is recruited to the leading edge of migrating cells where it colocalizes with actin and alphav-integrin, known mediators of motility and adhesion. Knockdown of CD155 also altered cellular morphology, resulting in cells that were larger and more elongated than controls when plated on a Matrigel substrate.
Conclusion: These results implicate a role for CD155 in mediating tumor cell invasion and migration and suggest that CD155 may contribute to tumorigenesis.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC524493 | PMC |
| http://dx.doi.org/10.1186/1471-2407-4-73 | DOI Listing |
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