IncI1 plasmid R64 encodes type IV pili or thin pili, which contain PilV adhesins. The C-terminal segments of PilV adhesins are exchanged into seven types by shufflon multiple DNA inversion. PilV adhesins determine recipient specificity in R64 liquid matings through the recognition of lipopolysaccharides (LPSs) on the surface of recipient cells. Using various waa mutants of Escherichia coli R1 as recipient cells, liquid mating experiments suggest that PilVA adhesin recognizes the GlcNAc(beta1-3)Glc moiety of E.coli R1 type LPS. The direct binding of PilV adhesins to LPSs of the recipient bacterial strains was demonstrated using filter overlay assays. The specificity of PilV-LPS binding is in close agreement with the recipient specificity determined by R64 liquid matings. The C-terminal segments of PilVA, PilVC, PilVC', and PilVD' adhesins were expressed as fusion proteins with glutathione-S-transferase (GST). GST-A, GST-C, GST-C', and GST-D' proteins bound to their respective LPSs with the specificities identical with those determined in the R64 liquid matings, indicating that the C-terminal segments of PilV adhesins bind to specific moieties of LPS molecules.
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Systematic investigation of recipient cell genetic requirements reveals important surface receptors for conjugative transfer of IncI2 plasmids.
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Département de biologie, Faculté des sciences, Université de Sherbrooke, Sherbrooke, QC, J1K 2R1, Canada.
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January 2023
College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Antimicrobial resistance has become a major public health issue of global concern. Conjugation is an important way for fast spreading drug-resistant plasmids, during which the type Ⅳ pili plays an important role. Type Ⅳ pili can adhere on the surfaces of host cell and other medium, facilitating formation of bacterial biofilms, bacterial aggregations and microcolonies, and is also a critical factor in liquid conjugation.
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Département de biologie, Faculté des sciences, Université de Sherbrookegrid.86715.3d, Sherbrooke, Quebec, Canada.
Type IV pili (T4P) are common bacterial surface appendages involved in different biological processes such as adherence, motility, competence, pathogenesis, and conjugation. In this work, we describe the T4P of TP114, an IncI2 enterobacterial conjugative plasmid recently shown to disseminate at high rates in the mouse intestinal tract. This pilus is composed of the major PilS and minor PilV pilins that are both important for conjugation in broth and in the gut microbiota but not on a solid support.
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Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, North Carolina, USA.
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Faculté de Médecine, Université de Paris, Paris 75006, France;
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