AI Article Synopsis
- Recent efforts aim to enhance the efficiency of fluorescence immunoassays by using micro- or nanocrystalline dyes instead of traditional labeling molecules.
- The study explores the use of amphiphilic polymers as a simpler alternative to the complex layer-by-layer assembly method, which typically involves polyelectrolytes and biorecognition molecules like immunoglobulins.
- It evaluates how factors like surface charge and particle size affect the performance of these immunoassays, revealing important insights for improving their effectiveness.
Article Abstract
Recently, numerous attempts have been made to improve the performance of fluorescence immunoassays. One way pursued is the substitution of labeling molecules by micro- or nanocrystalline dyes. The surfaces of these particulate structures are typically engineered by a layerwise assembly of oppositely charged polyelectrolytes, the outer layer being constituted of biorecognition molecules, for example, immunoglobulins. In this study, we show that amphiphilic polymers such as alkylated poly(ethylene imine)s and 1,2-distearoyl-sn-glycero-3-phosphatoethanolamine-N-[amino(poly(ethylene glycol))] can fully substitute the more intricate layer-by-layer technique and evaluate the influence of surface charge and particle size on the overall performance of these assays.
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| http://dx.doi.org/10.1021/la0486032 | DOI Listing |
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