Bioprocess equipment: characterization of energy dissipation rate and its potential to damage cells.

A study was conducted in which analytical, computational, and experimental measurements combined with analysis were made to characterize the local energy dissipation rate in a variety of conditions, vessels, and geometries that animal cells would encounter in typical bioprocessing situations. With no gas-liquid interfaces present, as expected, the local energy dissipation rate is typically orders of magnitude lower than what has been experimentally demonstrated to catastrophically damage typically used, suspended animal cells. However, local energy dissipation rates shown to remove animal cells from microcarriers are achievable under some normal operating conditions and geometries. Whether local energy dissipation rates created under typical operating conditions can have nonlethal effects is still an open question and currently under investigation. Whether the sensitivity of other, nontypical, suspended animal cells such as cells obtained directly from tissue (primary cells) and clusters of cells, such as islets, are more sensitive than the typically used cells is also still under investigation.