AI Article Synopsis
- The study aimed to evaluate the effects of the human endostatin (hES) gene on tongue squamous cell carcinoma (TSCC) growth and its expression in a mouse model.
- Researchers used Lipofectamine to transfer the hES gene into Tca8113 cells and observed the resulting tumor growth in mice, noting changes in protein expression levels and tumor characteristics.
- Results showed that hES gene expression significantly inhibited tumor growth, lowered vascular endothelial growth factor (VEGF) levels, reduced microvessel density, and increased apoptosis in the cancer cells, indicating its potential as a therapeutic target.
Article Abstract
Objective: To investigate the expression of transfected human endostatin (hES) gene in tongue squamous cell carcinoma (TSCC) and its inhibitory effects on the growth of tumor cells in vivo.
Methods: Lipofectamine-mediated hES gene was transferred into Tca8113 cells, selected with Blasticidin S; The stable transfected cells were inoculated in BALB/c mice, and then the growth of xenografts was observed. The hES and vascular endothelial growth factor (VEGF) protein expression of xenografts was detected by S-P immuno-histochemical assay. We also detected the microvessel density (MVD) of xenografts with Weidern's method and apoptotic index of the tumor cells by flow cytometry (FCM).
Results: The hES protein expression of xenografts in experimental group was significantly higher than that in control group (P < 0.01), while the expression of VEGF protein was on the other way round (P < 0.01). MVD counting of xenografts in experimental group was lower than that in control group (P < 0.01). The mean apoptotic level of the tumor cells in control group was also lower than in experimental group (P < 0.01). In addition, the inhibitory rate to growth of xenografts induced by hES transfection was 78.9%.
Conclusions: hES gene can be transferred into TSCC cells and then induce corresponding protein expression efficiently in xenograft model, resulting in significantly inhibitory effects on the xenografts in vivo.
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