AI Article Synopsis
- Foamy viruses (FVs) have a protein called transcriptional transactivator (Tas) that activates gene expression through specific promoter regions.
- The study focused on the feline foamy virus (FFV) Tas protein, which is less similar to non-human primate versions, and found that FFV Tas specifically enhances gene expression from its own promoters without affecting others like HIV-1.
- Key binding sites for FFV Tas were identified in the 5' long terminal repeat (LTR), indicating that it interacts with multiple regions of the virus's DNA to regulate transcription.
Article Abstract
Foamy viruses (FVs) harbour a transcriptional transactivator (Tas) and two Tas-responsive promoter regions, one in the 5' long terminal repeat (LTR) and the other an internal promoter (IP) in the envelope gene. To analyse the mechanism of transactivation of the FVs, the specificity of feline FV (FFV) Tas protein, which is more distantly related to the respective proteins of non-human primate origin, were investigated. FFV Tas has been shown specifically to activate gene expression from the cognate promoters. No cross-transactivation was noted of the prototype foamy virus and human immunodeficiency virus type 1 LTR. The putative transactivation response element of FFV Tas was mapped to the 5' LTR U3 region (approximately nt -228 to -195). FFV Tas binds to this element in addition to a previously described sequence (position -66 to -51). It is therefore concluded that FFV Tas is a DNA-binding transactivator that interacts with at least two regions in the virus LTR.
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| http://dx.doi.org/10.1099/vir.0.80088-0 | DOI Listing |
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