Objective: To investigate the effects of recombinant Helicobacter pylori catalase (rHpCAT)on oxidative stress in rat colonic mucosal epithelial cells.
Methods: Oxidative stress model was established by hydroxyl generated from Fenton reaction in cultured colonic mucosal epithelial cells isolated from normal rats, in the model of which the effects of rHpCAT were observed. The cells were divided into normal control, model, 5-aminosalicylic acid (5-ASA, 0.1 mmol/L), and rHpCAT (1 x 10(5), 1 x 10(6), and 1 x 10(7) U/kg, respectively) groups. At the end of the experiment, the content of lactic dehydrogenase (LDH), malondialdehyde (MDA), myeloperoxidase (MPO), glutathione peroxidase (GSH-Px), catalase (CAT) and, superoxide dismutase (SOD) were detected in the culture supernatant.
Results: The contents of LDH, MDA and MPO were elevated while those of GSH-Px, CAT and SOD reduced in the model group. rHpCAT at different doses reduced the release of LDH, depressed the contents of MDA and MPO, and increased the contents of GSH-Px, SOD and CAT.
Conclusion: rHpCAT has protective effects against rat colonic mucosal oxidative damage.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Survey of the Actual Practices Used for Endoscopic Removal of Colon Polyps in Korea: A Comparison with the Current Guidelines.
Gut Liver
January 2025
Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
Background/aims: We investigated the clinical practice patterns of Korean endoscopists for the endoscopic resection of colorectal polyps.
Methods: From September to November 2021, an online survey was conducted regarding the preferred resection methods for colorectal polyps, and responses were compared with the international guidelines.
Results: Among 246 respondents, those with <4 years, 4-9 years, and ≥10 years of experience in colonoscopy practices accounted for 25.
The Relationship Between A High-Fat Diet, Gut Microbiome, and Systemic Chronic Inflammation: Insights from Integrated Multi-Omics Analysis.
Am J Clin Nutr
December 2024
Key Laboratory of Hepatosplenic Surgery, Ministry of Education, The First Affiliated Hospital of Harbin Medical University, Harbin, China. Electronic address:
Background: The detrimental effects of a high-fat diet (HFD) extend beyond metabolic consequences and include systemic chronic inflammation (SCI), immune dysregulation, and gut health disruption.
Objectives: In this study, we used Mendelian randomization (MR) to investigate the relationship between HFD and gut microbiota, and SCI.
Methods: Genetic variants associated with dietary fat were utilized to explore causal relationships.
Functionalization of almond gum through covalent and non-covalent interactions for biomedical applications.
Int J Biol Macromol
December 2024
Department of Chemistry, Himachal Pradesh University, Shimla 171005, India.
The versatile properties of carbohydrate polymers make them a relevant, promising precursor to design innovative materials for use in biomedical applications. Recent research mainly focuses on the development of the polysaccharide based functional materials. Hydrogel derived materials are a source of great motivation for the development of drug delivery (DD) carriers with inherent therapeutic potential.
View Article and Find Full Text PDFStructural Characterization of Water-Soluble Pectin from the Fruit of and Its Protective Effects against DSS-Induced Colitis in Mice.
J Agric Food Chem
January 2025
Institute of Agro-Products Processing Science and Technology, Sichuan Academy of Agricultural Sciences, Chengdu 610066 Sichuan, China.
Polysaccharides from L. were investigated for their structural characterization and anti-inflammatory activity. Four low polymer dispersity index (PDI) subfractions were obtained: DRP-1 (153.
View Article and Find Full Text PDFHistologic and Endoscopic Findings Are Highly Correlated in a Prospective Cohort of Patients With Inflammatory Bowel Diseases.
J Crohns Colitis
December 2024
Division of Gastroenterology, Department of Medicine, University of Miami - Leonard Miller School of Medicine, Miami, FL, USA.
Background And Aims: The advantages of endoscopic vs histologic assessments of inflammation in inflammatory bowel disease remain unclear. We compared endoscopic and histologic inflammation in a prospective cohort. Furthermore, in patients with discordant findings, we compared the ability of endoscopy vs histology to predict disease course.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!