Objective: To investigate the expression of phospholipase C-gamma1 (PLCG1) mRNA in rats during early postnatal period.
Methods: Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression of PLCG1 mRNA in 28 samples extracted from the liver, lung, kidney and brain of rats aged 1, 3, 5, 7 days, and 2, 3, 5 weeks. Specific PLCG1 product and GAPDH product as the internal control were both amplified by RT-PCR, and the ratio of their integral optical density was calculated to estimate the relative mRNA expression of PLCG1.
Results: PLCG1 was expressed in rat liver, lung, kidney and brain at the 7 postnatal time points, and the expression varied significantly with time and between the different organs (P<0.01), virtually undetectable in the liver on postnatal day 1 and reaching the highest level in the brain tissues on postnatal day 7.
Conclusion: The differences in PLCG1 expression in various organs and development periods suggest that PLCG1 is involved in cell proliferation and differentiation during the early development of rats.
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