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Silica hydrogels as a carbon-free solid media for the culture of diverse organisms.
FEMS Microbes
December 2024
Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester M1 7DN, United Kingdom.
Bacteriological agar plates are commonly used to carry out experiments for the selective growth of microorganisms and the isolation of single-strain colonies. However, the presence of agar itself may be a confounding factor since it may serve as a source of carbon and energy. Moreover, there have been ongoing constraints on the production and sourcing of agar.
View Article and Find Full Text PDFAn Image Processing Tool for Automated Quantification of Bacterial Burdens in Zebrafish Larvae.
Zebrafish
December 2024
Department of Medicine, Molecular Immunity Unit, Cambridge Institute of Therapeutic Immunology and Infectious Diseases, University of Cambridge, Cambridge, UK.
Zebrafish larvae are used to model the pathogenesis of multiple bacteria. This transparent model offers the unique advantage of allowing quantification of fluorescent bacterial burdens (fluorescent pixel counts [FPC]) by facile microscopical methods, replacing enumeration of bacteria using time-intensive plating of lysates on bacteriological media. Accurate FPC measurements require laborious manual image processing to mark the outside borders of the animals so as to delineate the bacteria inside the animals from those in the culture medium that they are in.
View Article and Find Full Text PDFAmoeba plate test with as an innovative tool for recovery from sputum samples: a proof-of-concept study.
Microbiol Spectr
January 2025
Centre National de Référence des Légionelles, Institut des Agents Infectieux, Hôpital de la Croix-Rousse, Hospices Civils de Lyon, Lyon, France.
Unlabelled: recovery from pulmonary samples is challenging due to the lack of a specific medium and the abundance of overgrown respiratory flora. This study aimed to compare the amoeba plate test (APT), an amoebic coculture with , with the axenic culture to recover from pulmonary samples. serial dilutions ( = 15 strains from seven species, concentrations ranging: 10-10 CFU/mL) in water and spiked overgrown sputa ( = 8) were simultaneously plated on agar with amoebic monolayer (APT) and without (control).
View Article and Find Full Text PDFAnalysis of a persistent outbreak with vancomycin-resistant Enterococcus faecium revealed the need for an adapted diagnostic algorithm.
J Hosp Infect
January 2025
Laboratory for Medical Microbiology and Medical Immunology, Meander Medical Centre, Amersfoort, The Netherlands.
Objectives: The study institute was challenged with an outbreak of different vancomycin-resistant Enterococcus faecium (VREfm), including vanA- and/or vanB-containing isolates. Remarkably, screening overnight enriched specimens using a vanA and vanB real-time polymerase chain reaction (PCR) gave positive results for vanB with very low cycle threshold values, whereas VREfm-specific enrichment cultures remained negative. This paper describes the analysis of the diagnostic results leading to adaptation of the diagnostic algorithm.
View Article and Find Full Text PDFEvaluation of usage of readily accessible Enterobacteriaceae differential and selective media for identifying Candida auris.
Diagn Microbiol Infect Dis
January 2025
Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Background: Candida auris is a drug-resistant fungal pathogen. Diagnosing it is challenging due to the need for modern techniques. This study aims to evaluate the usefulness of Enterobacteriaceae culture media in screening and identifying Candida auris, as those media are accessible in resource-limited laboratories.
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