Hybrid organic-inorganic monolithic stationary phase for acidic compounds separation by capillary electrochromatography.

A novel type of organic-inorganic hybrid porous silica-based monolithic stationary phase for capillary electrochromatography (CEC) has been developed by sol-gel chemistry combined with supramolecular template-based approach in a simple and rapid manner. Both chromatographic interaction and electrophoretic migration contribute to the separation of acidic compounds by the monolithic column. Eight organic acids were separated rapidly with column efficiency up to 267,000 theoretical plates/m. The influences of buffer concentration and organic modifier content on the separation have been investigated. In addition, the hybrid monolithic column was used to separate triterpenoids from Ganoderma lucidum.