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Organelles in the ointment: improved detection of cryptic mitochondrial reads resolves many unknown sequences in cross-species microbiome analyses.
ISME Commun
January 2024
University of Washington, Division of Biological Sciences, School of Science, Technology, Engineering, and Mathematics, Bothell, WA, United States.
The genomes of mitochondria and chloroplasts contain ribosomal RNA (rRNA) genes, reflecting their ancestry as free-living bacteria. These organellar rRNAs are often amplified in microbiome studies of animals and plants. If identified, they can be discarded, merely reducing sequencing depth.
View Article and Find Full Text PDFThe Wound-Healing Effect of a Novel Fibroblasts-Impregnated Hydroxyethylcellulose Gel in a Rat Full-Thickness Burn Model: A Preclinical Study.
Biomedicines
September 2024
Cell Technology Center Pokrovsky, 199066 St. Petersburg, Russia.
The objective of this study was to assess the efficacy of a cell-containing wound dressing based on fibroblasts in hydroxyethylcellulose (HEC) gel for the local treatment of deep partial-thickness and/or full-thickness skin burns in an animal model. The rats (male Wistar, n = 100) were subjected to a full-thickness thermal burn (16 cm). Radical necrectomy was performed one day after the burn.
View Article and Find Full Text PDFBenchmarking bacterial taxonomic classification using nanopore metagenomics data of several mock communities.
Sci Data
August 2024
Transversal activities in Applied Genomics, Sciensano, Brussels, Belgium.
Taxonomic classification is crucial in identifying organisms within diverse microbial communities when using metagenomics shotgun sequencing. While second-generation Illumina sequencing still dominates, third-generation nanopore sequencing promises improved classification through longer reads. However, extensive benchmarking studies on nanopore data are lacking.
View Article and Find Full Text PDFIntroduction The coronavirus 2019 pandemic highlighted virtual learning (VL) as a promising tool for medical education, yet its effectiveness in teaching clinical reasoning (CR) remains underexplored. Past studies have suggested VL can effectively prepare students for clinical settings. Informed by the Milestones of Observable Behaviours for CR (MOBCR) and whole-case theoretical frameworks, the Mock Wards (MW) program was created using a novel blended in-person learning (IPL) and VL platform.
View Article and Find Full Text PDFBackground: Pandemic conditions of system-wide strain are associated with increased rates of in-hospital cardiac arrest (IHCA). During normal times, medical-surgical nurses may forget cardiopulmonary resuscitation (CPR) skills as soon as 3 months after training, leaving them unprepared and anxious about managing cardiac arrests. During pandemic surges, heightened anxiety can also impact concentration and confidence.
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