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| http://dx.doi.org/10.1155/2004/868421 | DOI Listing |
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Oligo-FISH Paints in Triticeae.
Curr Protoc
February 2022
Center for Informational Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.
Article Synopsis
- Developed seven oligonucleotide pools targeting barley and wheat chromosomes to facilitate chromosomal Oligo-FISH painting methods.
- Utilized high-throughput karyotyping for over 350 species in the Triticeae tribe, including various genera like Triticum and Hordeum.
- The new Oligo-FISH technique aids in identifying chromosomes and establishing karyotypes, especially useful for wild Triticeae species with nonsequenced genomes.
Optimization of three FISH procedures for in situ detection of anaerobic ammonium oxidizing bacteria in biological wastewater treatment.
J Microbiol Methods
August 2009
Department of Microbiology, Technische Universität München, D-85354 Freising, Germany.
Fluorescence in situ hybridization (FISH) using fluorochrome-labeled DNA oligonucleotide probes has been successfully applied for in situ detection of anaerobic ammonium oxidizing (anammox) bacteria. However, application of the standard FISH protocols to visualize anammox bacteria in biofilms from a laboratory-scale wastewater reactor produced only weak signals. Increased signal intensity was achieved either by modifying the standard FISH protocol, using peptide nucleic acid probes (PNA FISH), or applying horse radish peroxidase- (HRP-) labeled probes and subsequent catalyzed reporter deposition (CARD-FISH).
View Article and Find Full Text PDFMYC translocation-negative classical Burkitt lymphoma cases: an alternative pathogenetic mechanism involving miRNA deregulation.
J Pathol
December 2008
Department of Human Pathology and Oncology, University of Siena, Italy.
The molecular feature of Burkitt lymphoma (BL) is the translocation that places c-Myc under the control of immunoglobulin gene regulatory elements. However, there is accumulating evidence that some cases may lack an identifiable MYC translocation. In addition, during the EUROFISH project, aiming at the standardization of FISH procedures in lymphoma diagnosis, we found that five cases out of 35 classic endemic BLs were negative for MYC translocations by using a split-signal as well as a dual-fusion probe.
View Article and Find Full Text PDFCytogenetic analysis of diploidy in cloned bovine embryos using an improved air-dry karyotyping method.
Theriogenology
June 2005
Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT 84322, USA.
Of the few published studies on the cytogenetic analyses of bovine nuclear transferred (NT) embryos, results differ between air-dry and fluorescent in situ hybridization (FISH) procedures. A modified air-dry procedure is reported in this study that provides more metaphase plates for analysis. Day 5 and Day 7 bovine NT embryos were cultured in colcemid-containing CR1aa for 10-12 or 16-18 h, then treated in hypotonic sodium citrate for 3-5 min.
View Article and Find Full Text PDFReal-time reverse transcription-PCR and fluorescence in-situ hybridization are complementary to understand the mechanisms involved in HER-2/neu overexpression in human breast carcinomas.
Histopathology
April 2005
Department of Pathology, University Hospital of K.U. Leuven, and Centre for Human Genetics, K.U. Leuven, Leuven, Belgium.
Aims: To evaluate the HER-2/neu status at the mRNA and DNA level of breast carcinomas and to compare it with HER-2/neu receptor overexpression by immunohistochemistry (IHC).
Methods And Results: In 32 invasive breast carcinomas, frozen tissue was available for real-time detection of HER-2/neu mRNA levels by reverse transcription-polymerase chain reaction (RT-PCR). Corresponding paraffin sections were examined by IHC and fluorescence in-situ hybridization (FISH).
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