The non-structural glycoprotein (SGP) of Ebola virus (EboV) is secreted in large amounts from infected cells as a disulfide-linked homodimer. In this communication, highly purified SGP, derived from Vero E6 cultures infected with the Zaire species of EboV, was used to determine the correct localization of inter- and intrachain disulfide bonds. Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry analysis of proteolytic cleavage fragments indicates that all cysteines (six per monomeric unit) form unique disulfide bonds. Monomers of the SGP homodimer are joined in a parallel manner by two intersubunit disulfide bonds formed between paired N-terminal and C-terminal cysteines (C53-C53' and C306-C306'). The remaining cysteines are involved in intrachain disulfide bonding (paired as C108-C135 and C121-C147), which resembles the disulfide bond topology of fibronectin type II domains. The findings presented here provide the foundation for future studies aimed at defining the structural and functional properties of SGP.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.bbrc.2004.08.148 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Supramolecular transparent plastic engineering covalent-and-supramolecular polymerization.
Mater Horiz
January 2025
College of Chemistry and Chemical Engineering, Hunan University, Hunan 410082, P. R. China.
Supramolecular glass and plastic are a new generation of artificial transparent materials that exhibit excellent optical behavior and processability. However, owing to inherent deficiencies in their mechanical toughness and long-term stability, supramolecular materials lack the potential for functionalization and application. Inspired by the toughening phenomena in biological systems, a synergistic covalent-and-supramolecular polymerization strategy was applied to construct plastic-like supramolecular materials with high transmittance the solvent-free one-pot amidation of thioctic acid and (poly)amines.
View Article and Find Full Text PDFPreparation and Characterization of a Novel Self-Healing Transparent Polyimide Film Based on Dynamic Disulfide Bonds.
Polymers (Basel)
December 2024
Department of Materials Engineering, Taiyuan Institute of Technology, Taiyuan 030008, China.
Self-healing optically transparent polyimides have potential applications in optoelectronic device fabrication. In this study, for the first time, we successfully prepared a novel self-healing polyimide film containing reversible disulfide bonds through chemical imidization by introducing cystamine as a self-healing functional monomer into the molecular structure of conventional polyimides. The incorporation of cystamine enabled the films to maintain high transmittance (>87%) and tensile strength (>99 MPa).
View Article and Find Full Text PDFDissecting Cytophagalysin: Structural and Biochemical Studies of a Bacterial Pappalysin-Family Metallopeptidase.
Biomolecules
December 2024
Synthetic Structural Biology Group, Molecular Biology Institute of Barcelona (IBMB), Spanish National Research Council (CSIC), 08028 Barcelona, Spain.
Cytophaga is a genus of Gram-negative bacteria occurring in soil and the gut microbiome. It is closely related to pathogenic spp. that cause severe diseases in fish.
View Article and Find Full Text PDFStretchable, Self-Healable, and Durable Conductive Elastomer Derived from a Rationally Designed Covalently Cross-Linked Network.
ACS Appl Mater Interfaces
January 2025
School of Medicine, Huanghe Science and Technology University, Zhengzhou 450061, P. R. China.
Silver nanowire (Ag NW)-based elastic conductors have been considered a promising candidate for key stretchable electrodes in wearable devices. However, the weak interface interaction of Ag NWs and elastic substrates leads to poor durability of electronic devices. For everyday usage, an additional self-healing ability is required to resist scratching and damage.
View Article and Find Full Text PDFMicrostructural Assessment of Molybdenum Disulfide Coatings Using Nanoindentation Hardness.
ACS Appl Mater Interfaces
January 2025
Material, Physical and Chemical Sciences Center, Sandia National Laboratories, Albuquerque, New Mexico 87123, United States.
MoS coatings are used extensively in aerospace and defense applications due to their ultralow friction and high wear resistance. Burnished and resin-bonded MoS coatings are commonly used in these applications due to simplicity in deposition and history of use, despite issues with consistency in coating properties and performance. Physical vapor deposition (PVD) of MoS thin films has emerged as a process alternative in the past 50 years, promising far greater control over film structure and composition but at a greater cost.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!