Aim: To prepare bispecific monoclonal antibody (bsmAb) against HIV p24 and human group A erythrocytes, and set up an indirect hemagglutination test for detecting HIV p24.
Methods: Hybridoma cells 2-E(4) secreting anti-HIV p24 mAb and hybridoma cells S(2) secreting anti-human group A erythrocyte mAb were naturalized with 8-Ag and 5-BrdU respectively, making them sensitive to HAT. Then the two hybridoma cells sensitive to HAT were fused by routine method and hybrid-hybridoma cells secreting the bsmAb against HIV p24 and human group A erythrocytes were screened. The bsmAb was used to establish an indirect hemagglutination test for detecting HIV p24.
Results: 6 hybrid-hybridoma cell lines were obtained. An indirect hemagglutination test for detecting HIV p24 was set up by using the bsmAb, and its sensitivity reached 400 ng/L.
Conclusion: The bsmAb against HIV p24 and human group A erythrocytes is prepared and a rapid indirect hemagglutination test for detecting HIV p24 is developed by using the purified bsmAb.
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