In this paper, the spatial construction models of populations M. persicae and its predatory natural enemy E. graminicola during different periods were simulated by geostatistics, and their spatial relationships were analyzed. The spatial structure of M. persicae population was described by spherical model, showing an aggregated spatial arrangement. Its spatial dependence was 2.0252-4.1495 m, heterogeneity degree was 10,281.36-300,216.30, and sample variance was 12,176.81-303,433.70. The spatial structure of E . graminicola population was also simulated by spherical model, showing an aggregated spatial arrangement. Its spatial dependence was 3.7328-4.8983 m, heterogeneity degree was 1.4482-4.4134, and sample variance was 1.6941-5.8167. The results and methods could be applied to monitor the temporal and spatial dynamics of target insect pest population in tobacco field, and provide scientific basis for ecological control.

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