The human beta-globin locus control region DNase I hypersensitive site-2 (LCR HS-2) is erythroid-specific and is located 10.9 kb upstream of the epsilon-globin gene. Most studies have only examined the core region of HS-2. However, previous studies in this laboratory indicate that positioned nucleosomes are present at the 5'- and 3'-flanking regions of HS-2. In addition, footprints were observed that indicated the involvement of DNA-binding proteins in positioning the nucleosome cores. A consensus GATA-1 site exists in the region of the 3'-footprint. In this study, using an electrophoretic mobility shift assay (EMSA) and DNase I footprinting, we confirmed that GATA-1 binds in vitro at the 3'-end of HS-2. An additional GATA-1 site was found to bind GATA-1 in vitro at a site positioned 40 bp upstream. At the 5'-end of HS-2, DNase I footprinting revealed a series of footprints showing a marked correlation with the in vivo footprints. EMSA indicated the presence of several erythroid-specific complexes in this region including GATA-1 binding. Sequence alignment for 12 mammalian species in HS-2 confirmed that the highest conservation to be in the HS-2 core. However, a second level of conservation extends from the core to the sites of the proposed positioning proteins at the HS-2 flanking regions, before declining rapidly. This indicates the importance of the HS-2 flanking regions and supports the proposal of nucleosome positioning proteins in these regions.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.bbaexp.2004.04.002 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A novel approach for efficient co-expression of two foreign genes based on the reverse genetic system of Newcastle disease virus.
Front Microbiol
December 2024
Department of Epidemiology, School of Public Health, Harbin Medical University, Harbin, China.
Article Synopsis
- Newcastle disease virus (NDV) serves as a valuable model for studying viral mechanisms and is being utilized as a vector for vaccine development and cancer therapy.
- A reverse genetic system was created for the NDV Mukteswar strain, allowing for the insertion of the enhanced green fluorescent protein (EGFP) gene between the P and M genes to form a stable transcription unit that expresses exogenous genes over multiple generations.
- Through this system, a recombinant virus that co-expresses EGFP and cherry fluorescent protein (CFP) was developed, demonstrating efficient simultaneous expression of both proteins, though with slight differences in expression levels, and similar growth rates with mild virulence attenuation compared to the original strains.
Paranode length in the prefrontal cortex of subjects with major depression and rats under chronic unpredictable stress.
J Affect Disord
December 2024
Department of Psychiatry and Human Behavior, University of Mississippi Medical Center, USA.
Experimental studies of major depressive disorder (MDD) and stress reveal connectivity disturbances of the prefrontal cortex (PFC) that may involve molecular and morphological changes in myelin and the axons it enwraps. These alterations may also affect the nodes of Ranvier (NR), myelin-bare axon stretches along myelin sheaths necessary for action potential propagation, as well as the paranodes, specialized regions of the myelin sheath flanking NRs. Thus, we investigated whether paranode length and the labeling of paranode marker CASPR in PFC white matter (WM) differed in MDD subjects and chronic stress-exposed rats, as compared to their respective controls.
View Article and Find Full Text PDFA scaleable inducible knockout system for studying essential gene function in the malaria parasite.
Nucleic Acids Res
December 2024
Malaria Biochemistry Laboratory, The Francis Crick Institute, 1 Midland Road, NW1 1AT London, UK.
The malaria parasite needs nearly half of its genes to propagate normally within red blood cells. Inducible ways to interfere with gene expression like the DiCre-lox system are necessary to study the function of these essential genes. However, existing DiCre-lox strategies are not well-suited to be deployed at scale to study several genes simultaneously.
View Article and Find Full Text PDFEfficient identification of genomic insertions and surrounding regions in two transgenic maize events using third-generation single-molecule nanopore sequencing technology.
Sci Rep
December 2024
Institute of Agricultural Biotechnology/Institute of Agricultural Quality Standard and Testing Technology, Jilin Academy of Agricultural Sciences (Northeast Innovation Center of Agricultural Science and Technology in China), Changchun, China.
The increasing development of new genetically modified organisms underscores the critical need for comprehensive safety assessments, emphasizing the significance of molecular evidence such as gene integration, copy numbers, and adjacent sequences. In this study, the maize nitrate-efficient utilization gene ZmNRT1.1 A was introduced into maize variety y822 using transgenic technology, producing transgenic maize events ND4401 and ND4403 with enhanced tolerance to low nitrogen stress.
View Article and Find Full Text PDFMolecular Characterization of Dermatophytes Isolated in a Tertiary Care Hospital in the North Eastern Part of India.
Cureus
November 2024
Microbiology, North Eastern Indira Gandhi Regional Institute of Health and Medical Sciences, Shillong, IND.
Introduction Dermatophytes are the most common cause of superficial fungal infection. They are mostly diagnosed using phenotypic methods, but recently the molecular methods seem to be gaining ground. The objective of the present study was to compare the phenotypic and genotypic methods of identification of dermatophytes and understand the feasibility of using molecular methods for routine diagnosis of dermatophytosis.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!