Phospholipase Cepsilon (PLCepsilon) is a novel class of phosphoinositide-specific PLC with unknown physiological functions. Here, we present the first genetic analysis of PLCepsilon in an intact organism, the nematode Caenorhabditis elegans. Ovulation in C. elegans is dependent on an inositol 1,4,5-trisphosphate (IP(3)) signaling pathway activated by the receptor tyrosine kinase LET-23. We generated deletion mutants of the gene, plc-1, encoding C. elegans PLCepsilon. We observed a novel ovulation phenotype whereby oocytes are trapped in the spermatheca due to delayed dilation of the spermatheca-uterine valve. The expression of plc-1 in the adult spermatheca is consistent with its involvement in regulation of ovulation. On the other hand, we failed to observe genetic interaction of plc-1 with let-23-mediated IP(3) signaling pathway genes, suggesting a complex mechanism for control of ovulation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ydbio.2004.06.024 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Polymorphisms of and Genes and Their Correlation Analysis with Growth Traits in Ashidan Yaks.
Animals (Basel)
December 2024
Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Key Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou 730050, China.
The tyrosine protein kinase () gene, as a member of the non-receptor tyrosine kinase Tec family, plays a vital role in signal transduction mediation. Phospholipase C epsilon 1 (), a membrane-associated enzyme, is of paramount importance for the differentiation of myoblasts and the normal functioning of muscle tissue. In recent years, both of these genes have been reported to be associated with the economic traits of animals.
View Article and Find Full Text PDFRoles for PKC signaling in chromaffin cell exocytosis.
Biophys J
December 2024
Department of Neurosciences, University of Toledo, Toledo, Ohio. Electronic address:
Chromaffin cells of the adrenal medulla have an important role in the sympathetic stress response. They secrete catecholamines and other hormones into the bloodstream upon stimulation by the neurotransmitter pituitary adenylate cyclase-activating polypeptide (PACAP). PACAP causes a long-lasting and robust secretory response from chromaffin cells.
View Article and Find Full Text PDFPhospholipase Cε (PLCε) cleaves phosphatidylinositol lipids to increase intracellular Ca and activate protein kinase C (PKC) in response to stimulation of cell surface receptors. PLCε is activated via direct binding of small GTPases at the cytoplasmic leaflets of cellular membranes. In the cardiovascular system, the RhoA GTPase regulates PLCε to initiate a cardioprotective pathway, but the underlying molecular mechanism is not known.
View Article and Find Full Text PDFTensions on the actin cytoskeleton and apical cell junctions in the spermatheca are influenced by spermathecal anatomy, ovulation state and activation of myosin.
Front Cell Dev Biol
October 2024
Department of Biology, Northeastern University, Boston, MA, United States.
Article Synopsis
- Cells generate mechanical forces primarily through myosin acting on the actin cytoskeleton, particularly in the spermatheca where they drive muscle-like contractions during oocyte fertilization.
- Researchers used advanced techniques like laser ablation and RNA interference to investigate how myosin activity affects force distribution and tissue response in spermathecal cells.
- Findings indicated that while tension exists in the basal actomyosin fibers due to embryo presence, altering myosin activity affects both basal and apical tensions, suggesting a complex interaction between these forces.
DOT1L protects against podocyte injury in diabetic kidney disease through phospholipase C-like 1.
Cell Commun Signal
October 2024
Department of Endocrinology, The Second Affiliated Hospital of Zhejiang University School of Medicine, 88 Jiefang Road, Hangzhou, Zhejiang, China.
Background: Podocyte injury causes proteinuria and accelerates glomerular sclerosis during diabetic kidney disease (DKD). Disruptor of telomeric silencing 1-like (DOT1L), an evolutionarily conserved histone methyltransferase, has been reported in preventing kidney fibrosis in chronic kidney disease models. However, whether DOT1L exerts beneficial effects in diabetes induced podocyte injury and the underlying molecular mechanisms need further exploration.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!