[The long-term effects of rhBMP-2 and rhbFGF on alkaline phosphatase of bone mesenchymal stem cells].

Purpose: To evaluate the long-term effects of rhBMP-2 and rhbFGF on alkaline phosphatase(ALP) of rabbit bone mesenchymal stem cells by using them alone, associatedly and sequentially.

Methods: Under cell culture technique, ALP kits were used to evaluate ALP level of bone mesenchymal stem cells so as to reflect differentiation of the cells on the 2nd, 5th, 7th, and 10th day by adding rhBMP-2 and rhbFGF on different ways.

Results: Statistical analysis showed that rhBMP-2 could enhance while rhbFGF could inhibit the ALP level of rabbit bone mesenchymal stem cells significantly in long term, and also in a dose-dependent manner. The effects of using rhBMP-2 alone was similar to that of using the two growth factors associatedly, and they were significantly higher than that of using rhbFGF alone and using them sequentially.

Conclusion: Different growth factor has different long-term effect on differentiation of bone mesenchymal stem cells by adding them on different ways.