The comparative analysis of the production of the main pathogenicity factors by toxigenic and non-toxigenic clones of V. cholerae natural classical strain Dacca 35 Ogawa has been carried out. The data obtained in this analysis indicate that the appearance of turbid colonies, not synthesing cholera toxin, is linked with the production of an exopolysaccharide layer on the outer surface of the cells, which determines their morphology. The suggestion has been made that the regulatory gene toxR controls the expression of not only cholera toxin, protein OmpU, but also exopolysaccharide.
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Zh Mikrobiol Epidemiol Immunobiol
March 2005
The comparative analysis of the production of the main pathogenicity factors by toxigenic and non-toxigenic clones of V. cholerae natural classical strain Dacca 35 Ogawa has been carried out. The data obtained in this analysis indicate that the appearance of turbid colonies, not synthesing cholera toxin, is linked with the production of an exopolysaccharide layer on the outer surface of the cells, which determines their morphology.
View Article and Find Full Text PDFConjugational-mating experiments showed mrh genes coding for the synthesis of mannose and fucose resistant hemagglutinin/adhesin and vibrio motility gene mot to be localized in the chromosome of V. cholerae Dacca strain 35 close to tox-2 mutation providing a high level of toxin production and rfb locus which controls 01 antigen synthesis. The results indicate the presence of a block of virulence genes within ura-pur chromosomal region of V.
View Article and Find Full Text PDFEur J Clin Microbiol Infect Dis
January 1993
Laboratory Science Division, International Centre for Diarrhoeal Disease Research, Dacca, Bangladesh.
A rapid and sensitive method for the detection of Shigella dysenteriae type 1 and Shigella flexneri serotypes in faeces based on capture of the bacteria with immunomagnetic particles is described. The particles were coated with either of two different monoclonal antibodies specific for the O-antigens of Shigella dysenteriae type 1 and Shigella flexneri serotypes. Captured bacteria were detected by an enzyme immunoassay with O-antigen specific rabbit antiserum.
View Article and Find Full Text PDFZh Mikrobiol Epidemiol Immunobiol
March 1990
The study of 27 V. cholerae strains, isolated from cholera patients and found to be hemolytically inactive, with a view to establish their capacity for the production of cholera toxin has revealed that 4 strains (V. cholerae cholerae Dacca 35, V.
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