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| http://dx.doi.org/10.1128/JB.186.18.5973-5977.2004 | DOI Listing |
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Cryopreservation of bovine sperm causes single-strand DNA breaks that are localized in the toroidal regions of chromatin.
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Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology, University of Girona, S17003, Girona, Spain.
Article Synopsis
- - The study investigates sperm cryopreservation in cattle, focusing on the types and locations of DNA damage that occur during the freezing and thawing process, particularly whether this damage includes single-strand or double-strand breaks in the DNA.
- - Results showed a significant increase in single-strand breaks post-cryopreservation, while double-strand breaks remained consistent before and after the freezing process, indicating that cryopreservation primarily affects DNA integrity through single-strand breakage.
- - The research also found that DNA fragments shortened after cryopreservation predominantly come from regions linked to protamines, suggesting cryopreservation negatively impacts these specific parts of the sperm DNA, which is crucial for successful artificial insemination.
Gene therapy by virus-like self-spooling toroidal DNA condensates for revascularization of hindlimb ischemia.
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Department of Gastric Surgery, Cancer Hospital of China Medical University, No. 44 Xiaoheyan Road, Dadong District, Shenyang City, Liaoning, 110042, China.
Peripheral arterial diseases (PAD) have been reported to be the leading cause for limb amputations, and the current therapeutic strategies including antiplatelet medication or intervene surgery are reported to not clinically benefit the patients with high-grade PAD. To this respect, revascularization based on angiogenetic vascular endothelial growth factor (VEGF) gene therapy was attempted for the potential treatment of critical PAD. Aiming for transcellular delivery of VEGF-encoding plasmid DNA (pDNA), we proposed to elaborate intriguing virus-like DNA condensates, wherein the supercoiled rigid micrometer-scaled plasmid DNA (pDNA) could be regulated in an orderly fashion into well-defined nano-toroids by following a self-spooling process with the aid of cationic block copolymer poly(ethylene glycol)-polylysine at an extraordinary ionic strength (NaCl: 600 mM).
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TDP-43 forms aggregates in the neurons of patients with several neurodegenerative diseases. Human TDP-43 also aggregates and is toxic in yeast. Here, we used a yeast model to investigate (1) the nature of TDP-43 aggregates and (2) the mechanism of TDP-43 toxicity.
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The next generation of anticancer agents are emerging from rationally designed nanostructured materials. This work involved the synthesis and characterization of novel hollow DNA-conjugated gold nanoparticles (DNA-AuNPs) for controlled drug delivery. Polyethyleneimine (PEI) was bound to AuNPs, forming polymer-shell nanoparticles.
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