Background: To establish a genechip method for detection of hepatitis B virus (HBV) DNA, basal core promotor (BCP), and Pre-C mutants.
Methods: This study used two kinds of technology (PCR, oligochip), which can detect five mutant hotspots including nt 1 896, nt 1 899, nt 1 862, nt 1 764 and nt 1 762. The results of genechip method was verified by DNA sequencing.
Results: In detecting HBV DNA, the results of genechip were 100% consistent with those of DNA sequencing. In detecting HBV BCP and Pre-C mutants, 146 codons showed the same results using both methods, except for only 4 codons (P greater than 0.05).
Conclusion: This convenient high throughput genechip method could detect several BCP and Pre-C mutant codons at the same time. These results suggest that genechip method has the same positive rate and specificity with DNA sequencing method. It has more advantages than the latter in detecting mixed mutants and therefore may be used in clinical practice.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Genome-Wide microRNA Expression Profiling in Human Spermatozoa and Its Relation to Sperm Quality.
Genes (Basel)
January 2025
Institute for Regenerative Medicine and Biotherapy (IRMB), University of Montpellier, INSERM, CHU Montpellier, 34295 Montpellier, France.
Background: Sperm samples are separated into bad and good quality samples in function of their phenotype, but this does not indicate their genetic quality.
Methods: Here, we used GeneChip miRNA arrays to analyze microRNA expression in ten semen samples selected based on high-magnification morphology (score 6 vs. score 0) to identify miRNAs linked to sperm phenotype.
Transcriptomic analysis of pancreatic tissue from humans and mice identifies potential gene signatures and unexplored pathways during progression from acute to chronic pancreatitis.
Gene
March 2025
Translational Research Centre, Asian Healthcare Foundation, AIG Hospitals, Hyderabad, India. Electronic address:
Background: A comprehensive understanding of the molecular pathogenesis of chronic pancreatitis (CP), a fibroinflammatory disorder of the pancreas, is warranted for the development of targeted therapies. The current study focused on comparing the transcriptomes of pancreatic tissues obtained from patients with CP with those of two rodent models of chemically induced CP to identify dysregulated genes/signaling pathways.
Methods: Pancreatitis was induced in mice using cerulein and L-arginine.
Discovery of RNA Biomarkers for Prostate Cancer Using Cross-Platform Transcriptomics.
Int J Mol Sci
November 2024
Department of Urology, Radboud University Medical Centre, 6525 GA Nijmegen, The Netherlands.
Microarray and Single-Molecule Molecular Inversion Probe (smMIP)-based targeted RNA sequencing are two RNA profiling platforms for identifying disease-associated biomarkers. The microarray uses a GeneChip array with oligonucleotide probes to measure expression levels across thousands of genes, while smMIPs capture and quantify RNA transcripts and transcript variants via next-generation sequencing. To evaluate the strengths and weaknesses of both platforms, a comparative gene expression profiling study was conducted using RNA samples from 52 prostate tissues (normal, benign prostatic hyperplasia (BPH) and various prostate cancer (PCa) grades).
View Article and Find Full Text PDFExtracellular vesicles derived from bone marrow mesenchymal stem cells ameliorate liver fibrosis via micro-7045-5p.
Mol Cell Biochem
November 2024
School of Traditional Chinese Medicine, Capital Medical University, Beijing, China.
Introduction: Liver fibrosis is a crucial pathological factor in the persistence and progression of chronic liver disease. Increasing evidence has demonstrated the significant potential of extracellular vesicles (EVs) secreted by bone marrow mesenchymal stem cells (BMSCs) in the clinical treatment of liver fibrosis. This study aimed to mechanistically investigate the impact of BMSC-derived EVs (BMSC-EVs) containing miR-7045-5p on the autophagy of activated hepatic stellate cells (HSCs) during liver fibrosis.
View Article and Find Full Text PDFValidation of a genome-based model for adjusting radiotherapy dose (GARD) in patients with locally advanced rectal cancer.
Sci Rep
September 2024
Department of Radiation Oncology, Fujian Medical University Union Hospital, Fuzhou, Fujian Province, People's Republic of China.
Article Synopsis
- Neoadjuvant radiotherapy is the standard treatment for locally advanced rectal cancer, but its effectiveness varies among patients, prompting the need for personalized treatment approaches based on molecular signatures.
- The study aimed to develop and validate a genome-based model (GARD) for determining radiation doses in these patients by analyzing gene expression from tumors collected from 64 patients undergoing treatment between 2015 and 2018.
- Results indicated significant differences in treatment response, with a median NAR score of 8.43 demonstrating the variability in clinical efficacy among patients with similar tumor stages, highlighting the potential for tailored radiation therapy.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!