The effects of copper (Cu2+) and Cu-EDTA complex on the induction of HSP70 in the fish brain were studied. After the fish (Carassius auratus) were exposed to the experimental concentrations for forty days, the SDS-PAGE and Western Blotting techniques were used to detect the expression level of HSP70 in the fish brain. The results showed that Cu2+ and Cu-EDTA complex can induce the expression of HSP70 significantly. Comparing with the test groups exposed to Cu2+, the Cu-EDTA complex had changed the bioavailability and decreased the toxicity of the heavy metal. It was found that the fish exposed to the concentration of Cu2+ which below the national fishery water quality standard of 0.01 mg/L , also had a significant increase in the expression level of HSP70. Therefore, HSP70 as an environmental biomarker can be a more sensitive and rapid indicator than conventional end points in the aquatic ecosystem.
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